Goshima Gohta, Wollman Roy, Goodwin Sarah S, Zhang Nan, Scholey Jonathan M, Vale Ronald D, Stuurman Nico
Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Science. 2007 Apr 20;316(5823):417-21. doi: 10.1126/science.1141314. Epub 2007 Apr 5.
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
中期纺锤体是一种具有中心对齐染色体的双极微管阵列，其形成是细胞遗传物质忠实分离的前提条件。通过对果蝇S2细胞进行全基因组RNA干扰筛选，我们鉴定出约200个有助于纺锤体组装的基因，其中一半以上是意想不到的。该筛选与各种二次检测相结合，为纺锤体微管的产生方式、中心体的定位方式以及中心粒、中心体和动粒的组装方式带来了新的见解。
