果蝇S2细胞有丝分裂纺锤体组装所需的基因。
Genes required for mitotic spindle assembly in Drosophila S2 cells.
作者信息
Goshima Gohta, Wollman Roy, Goodwin Sarah S, Zhang Nan, Scholey Jonathan M, Vale Ronald D, Stuurman Nico
机构信息
Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
出版信息
Science. 2007 Apr 20;316(5823):417-21. doi: 10.1126/science.1141314. Epub 2007 Apr 5.
Abstract
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
摘要
中期纺锤体是一种具有中心对齐染色体的双极微管阵列，其形成是细胞遗传物质忠实分离的前提条件。通过对果蝇S2细胞进行全基因组RNA干扰筛选，我们鉴定出约200个有助于纺锤体组装的基因，其中一半以上是意想不到的。该筛选与各种二次检测相结合，为纺锤体微管的产生方式、中心体的定位方式以及中心粒、中心体和动粒的组装方式带来了新的见解。
相似文献
1
Genes required for mitotic spindle assembly in Drosophila S2 cells.果蝇S2细胞有丝分裂纺锤体组装所需的基因。
Science. 2007 Apr 20;316(5823):417-21. doi: 10.1126/science.1141314. Epub 2007 Apr 5.
2
Mechanisms of Mitotic Spindle Assembly.有丝分裂纺锤体组装的机制。
Annu Rev Biochem. 2016 Jun 2;85:659-83. doi: 10.1146/annurev-biochem-060815-014528. Epub 2016 Apr 21.
3
BubR1 and CENP-E have antagonistic effects upon the stability of microtubule-kinetochore attachments in Drosophila S2 cell mitosis.在果蝇S2细胞有丝分裂过程中，BubR1和着丝粒蛋白E（CENP-E）对微管-动粒附着的稳定性具有拮抗作用。
Cell Cycle. 2007 Jun 1;6(11):1367-78. doi: 10.4161/cc.6.11.4271. Epub 2007 Jun 11.
4
Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.Augmin：一种纺锤体内不依赖中心体的微管生成所需的蛋白质复合体。
J Cell Biol. 2008 May 5;181(3):421-9. doi: 10.1083/jcb.200711053. Epub 2008 Apr 28.
5
Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis.在动物有丝分裂过程中，动粒驱动的动粒纤维形成有助于纺锤体组装。
J Cell Biol. 2004 Dec 6;167(5):831-40. doi: 10.1083/jcb.200407090. Epub 2004 Nov 29.
6
Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization.由盘状退化基因4编码的果蝇γ微管蛋白环复合物亚基的突变差异性地破坏中心体蛋白定位。
Genes Dev. 2000 Dec 15;14(24):3126-39. doi: 10.1101/gad.182800.
7
MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity.MAST/Orbit在微管-动粒附着中发挥作用，对染色体排列和纺锤体双极性的维持至关重要。
J Cell Biol. 2002 May 27;157(5):749-60. doi: 10.1083/jcb.200201101. Epub 2002 May 28.
8
A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint.γ-微管蛋白环复合物蛋白在纺锤体组装检查点中的非中心体依赖性作用。
Science. 2006 Oct 27;314(5799):654-7. doi: 10.1126/science.1132834.
9
Drosophila mars is required for organizing kinetochore microtubules during mitosis.果蝇Mars在有丝分裂期间对于组织动粒微管是必需的。
Exp Cell Res. 2008 Oct 15;314(17):3209-20. doi: 10.1016/j.yexcr.2008.08.004. Epub 2008 Aug 19.
10
FACTORS GOVERNING THE PATTERN OF SPINDLE MICROTUBULE REGROWTH AFTER TUBULIN DEPOLYMERIZATION.微管蛋白解聚后纺锤体微管再生长模式的调控因素
Tsitologiia. 2016;58(4):299-303.
引用本文的文献
1
A brief historical perspective on cell cycle control of CENP-A assembly and inheritance.着丝粒蛋白A（CENP-A）组装与遗传的细胞周期控制简史
Chromosome Res. 2025 Jul 26;33(1):15. doi: 10.1007/s10577-025-09774-2.
2
Stage-specific transcriptomic analysis reveals insights into the development, reproduction and biological function of allergens in the European house dust mite Dermatophagoides pteronyssinus.阶段特异性转录组分析揭示了欧洲屋尘螨（粉尘螨）变应原的发育、繁殖和生物学功能。
BMC Genomics. 2025 May 26;26(1):527. doi: 10.1186/s12864-025-11703-w.
3
Cryo-EM structures of the Plant Augmin reveal its intertwined coiled-coil assembly, antiparallel dimerization and NEDD1 binding mechanisms.植物γ-微管蛋白环状复合物的冷冻电镜结构揭示了其相互缠绕的卷曲螺旋组装、反平行二聚化及NEDD1结合机制。
bioRxiv. 2025 Feb 27:2025.02.25.640204. doi: 10.1101/2025.02.25.640204.
4
CAMSAP2 is required for bridging fiber assembly to ensure mitotic spindle assembly and chromosome segregation in human epithelial Caco-2 cells.在人上皮Caco-2细胞中，桥连纤维组装需要CAMSAP2以确保有丝分裂纺锤体组装和染色体分离。
PLoS One. 2025 Jan 9;20(1):e0308150. doi: 10.1371/journal.pone.0308150. eCollection 2025.
5
Amplified centrosomes-more than just a threat.扩增的中心体——不仅仅是威胁。
EMBO Rep. 2024 Oct;25(10):4153-4167. doi: 10.1038/s44319-024-00260-0. Epub 2024 Sep 16.
6
Generating CRISPR-edited clonal lines of cultured S2 cells.生成经CRISPR编辑的培养S2细胞克隆系。
Biol Methods Protoc. 2024 Aug 17;9(1):bpae059. doi: 10.1093/biomethods/bpae059. eCollection 2024.
7
Kinesins regulate the heterogeneity in centrosome clustering after whole-genome duplication.驱动蛋白调节全基因组复制后中心体聚类的异质性。
Life Sci Alliance. 2024 Jul 29;7(10). doi: 10.26508/lsa.202402670. Print 2024 Oct.
8
γ-TuRCs and the augmin complex are required for the development of highly branched dendritic arbors in Drosophila.γ-TuRCs 和 augmin 复合物对于果蝇中高度分支的树突棘的发育是必需的。
J Cell Sci. 2024 May 1;137(9). doi: 10.1242/jcs.261534. Epub 2024 May 10.
9
Patronin regulates presynaptic microtubule organization and neuromuscular junction development in .Patronin调节线虫中的突触前微管组织和神经肌肉接头发育。
iScience. 2024 Jan 18;27(2):108944. doi: 10.1016/j.isci.2024.108944. eCollection 2024 Feb 16.
10
A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.前期 CENP-C 的动态群体对于减数分裂染色体分离是必需的。
PLoS Genet. 2023 Nov 29;19(11):e1011066. doi: 10.1371/journal.pgen.1011066. eCollection 2023 Nov.
本文引用的文献
1
A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint.γ-微管蛋白环复合物蛋白在纺锤体组装检查点中的非中心体依赖性作用。
Science. 2006 Oct 27;314(5799):654-7. doi: 10.1126/science.1132834.
2
Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.在基于黑腹果蝇细胞的实验中与长双链RNA相关的脱靶效应证据。
Nat Methods. 2006 Oct;3(10):833-8. doi: 10.1038/nmeth935.
3
Prevalence of off-target effects in Drosophila RNA interference screens.果蝇RNA干扰筛选中脱靶效应的发生率。
Nature. 2006 Sep 21;443(7109):359-63. doi: 10.1038/nature05179. Epub 2006 Sep 10.
4
Flies without centrioles.没有中心粒的苍蝇。
Cell. 2006 Jun 30;125(7):1375-86. doi: 10.1016/j.cell.2006.05.025.
5
Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins.着丝粒DNA和动粒蛋白的系统发育与结构分析
Genome Biol. 2006;7(3):R23. doi: 10.1186/gb-2006-7-3-r23. Epub 2006 Mar 22.
6
Making microtubules and mitotic spindles in cells without functional centrosomes.在没有功能性中心体的细胞中制造微管和有丝分裂纺锤体。
Curr Biol. 2006 Mar 21;16(6):564-9. doi: 10.1016/j.cub.2006.01.053.
7
GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation.GCP-WD是中心体和染色质介导的微管成核所必需的一种γ-微管蛋白靶向因子。
Nat Cell Biol. 2006 Feb;8(2):137-47. doi: 10.1038/ncb1349. Epub 2005 Dec 25.
8
Length control of the metaphase spindle.中期纺锤体的长度控制
Curr Biol. 2005 Nov 22;15(22):1979-88. doi: 10.1016/j.cub.2005.09.054.
9
The kinetochore and cancer: what's the connection?动粒与癌症：有何关联？
Curr Opin Cell Biol. 2005 Dec;17(6):576-82. doi: 10.1016/j.ceb.2005.09.012. Epub 2005 Oct 17.
10
Kinetochore structure and function.动粒的结构与功能。
Trends Cell Biol. 2005 Nov;15(11):589-98. doi: 10.1016/j.tcb.2005.09.010. Epub 2005 Oct 7.
Zotero 插件