• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

PCR amplification of long DNA fragments.

作者信息

Ponce M R, Micol J L

机构信息

Division of Biology, California Institute of Technology, Pasadena 91125.

出版信息

Nucleic Acids Res. 1992 Feb 11;20(3):623. doi: 10.1093/nar/20.3.623.

DOI:10.1093/nar/20.3.623
PMID:1741307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310446/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3b5/310446/4b0d59ef893d/nar00077-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3b5/310446/4b0d59ef893d/nar00077-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3b5/310446/4b0d59ef893d/nar00077-0233-a.jpg

相似文献

1
PCR amplification of long DNA fragments.长DNA片段的聚合酶链式反应扩增
Nucleic Acids Res. 1992 Feb 11;20(3):623. doi: 10.1093/nar/20.3.623.
2
Production of PCR mimics for any semiquantitative PCR application.
Biotechniques. 1997 Jun;22(6):1060-2. doi: 10.2144/97226bm10.
3
Long-PCR amplification of human genomic DNA.人类基因组DNA的长片段PCR扩增
Methods Mol Biol. 2011;688:67-74. doi: 10.1007/978-1-60761-947-5_6.
4
PCR amplification, cloning, and sequencing of ancient DNA.古代DNA的聚合酶链式反应(PCR)扩增、克隆及测序
Methods Mol Biol. 2012;840:111-9. doi: 10.1007/978-1-61779-516-9_15.
5
Enzymatic amplification of DNA by PCR: standard procedures and optimization.通过聚合酶链式反应(PCR)进行DNA的酶促扩增:标准程序与优化
Curr Protoc Mol Biol. 2001 Nov;Chapter 15:Unit 15.1. doi: 10.1002/0471142727.mb1501s56.
6
Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing.Taq聚合酶催化的体外DNA扩增过程中的不完全引物延伸;用于DNA测序的应用
Nucleic Acids Res. 1989 Dec 11;17(23):9613-20. doi: 10.1093/nar/17.23.9613.
7
PCR-based assays for strand-specific measurement of DNA damage and repair. I. Strand-specific quantitative PCR.用于DNA损伤和修复链特异性测量的基于PCR的检测方法。I. 链特异性定量PCR
Methods Mol Biol. 1999;113:227-40. doi: 10.1385/1-59259-675-4:227.
8
Identification of transgenic mice carrying the CAT gene with PCR amplification.
Biotechniques. 1989 Nov-Dec;7(10):1065-70.
9
Long Fragment Polymerase Chain Reaction.长片段聚合酶链反应
Methods Mol Biol. 2017;1620:65-74. doi: 10.1007/978-1-4939-7060-5_3.
10
A gel electrophoresis loading system to prevent laboratory contamination by amplification products.一种防止实验室被扩增产物污染的凝胶电泳加样系统。
Biotechniques. 2014 Oct 1;57(4):199-200, 202. doi: 10.2144/000114216. eCollection 2014 Oct.

引用本文的文献

1
Comparative Analysis of Blood and Bone Marrow for the Detection of Circulating and Disseminated Tumor Cells and Their Prognostic and Predictive Value in Esophageal Cancer Patients.血液与骨髓用于检测食管癌患者循环肿瘤细胞和播散肿瘤细胞的比较分析及其预后和预测价值
J Clin Med. 2020 Aug 18;9(8):2674. doi: 10.3390/jcm9082674.
2
A ToxR homolog from Vibrio anguillarum serotype O1 regulates its own production, bile resistance, and biofilm formation.来自鳗弧菌血清型O1的一种ToxR同源物调控其自身的产生、胆汁抗性和生物膜形成。
J Bacteriol. 2002 Mar;184(6):1630-9. doi: 10.1128/JB.184.6.1630-1639.2002.
3
VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum.

本文引用的文献

1
Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011.
2
Ontogenic activation of a fusion gene introduced into sea urchin eggs.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):151-5. doi: 10.1073/pnas.84.1.151.
3
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.使用嗜热栖热菌DNA聚合酶进行DNA测序以及对聚合酶链反应扩增的DNA进行直接测序。
VanT是哈维氏弧菌LuxR的同源物,可调控鳗弧菌中丝氨酸、金属蛋白酶、色素和生物膜的产生。
J Bacteriol. 2002 Mar;184(6):1617-29. doi: 10.1128/JB.184.6.1617-1629.2002.
4
Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis.通过16S rRNA基因末端限制性片段分析评估美国西南部四种土壤中的微生物多样性。
Appl Environ Microbiol. 2000 Jul;66(7):2943-50. doi: 10.1128/AEM.66.7.2943-2950.2000.
5
Levels of bacterial community diversity in four arid soils compared by cultivation and 16S rRNA gene cloning.通过培养和16S rRNA基因克隆比较四种干旱土壤中细菌群落多样性水平。
Appl Environ Microbiol. 1999 Apr;65(4):1662-9. doi: 10.1128/AEM.65.4.1662-1669.1999.
6
Isolation of estrogen-responsive genes with a CpG island library.利用CpG岛文库分离雌激素反应基因。
Mol Cell Biol. 1998 Jan;18(1):442-9. doi: 10.1128/MCB.18.1.442.
7
A mutation in an HSP90 gene affects the sexual cycle and suppresses vegetative incompatibility in the fungus Podospora anserina.热休克蛋白90(HSP90)基因的突变会影响真菌嗜热栖热放线菌的有性生殖周期,并抑制其营养体不亲和性。
Genetics. 1997 Oct;147(2):581-8. doi: 10.1093/genetics/147.2.581.
8
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.在野生型大肠杆菌基因组中产生精确缺失和插入的方法:应用于开放阅读框表征
J Bacteriol. 1997 Oct;179(20):6228-37. doi: 10.1128/jb.179.20.6228-6237.1997.
9
Analysis of a chemotaxis operon from Rhodospirillum centenum.来自百脉根红螺菌的趋化操纵子分析。
J Bacteriol. 1997 Sep;179(18):5712-9. doi: 10.1128/jb.179.18.5712-5719.1997.
10
Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons.谷胱甘肽S-转移酶编码基因作为能够降解多环芳烃的环境细菌分离株的潜在探针。
Appl Environ Microbiol. 1997 Aug;63(8):3286-90. doi: 10.1128/aem.63.8.3286-3290.1997.
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40. doi: 10.1073/pnas.85.24.9436.
4
Improved yields of long PCR products using gene 32 protein.使用基因32蛋白提高长PCR产物的产量。
Nucleic Acids Res. 1990 Feb 25;18(4):1079. doi: 10.1093/nar/18.4.1079.