热休克蛋白90（HSP90）基因的突变会影响真菌嗜热栖热放线菌的有性生殖周期，并抑制其营养体不亲和性。
A mutation in an HSP90 gene affects the sexual cycle and suppresses vegetative incompatibility in the fungus Podospora anserina.
作者信息
Loubradou G, Bégueret J, Turcq B
机构信息
Laboratoire de Génétique Moléculaire des Champignons Filamenteux, CNRS UPR 9026, Bordeaux, France.
出版信息
Genetics. 1997 Oct;147(2):581-8. doi: 10.1093/genetics/147.2.581.
Abstract
Vegetative incompatibility is widespread in fungi but its molecular mechanism and biological function are still poorly understood. A way to study vegetative incompatibility is to investigate the function of genes whose mutations suppress this phenomenon. In Podospora anserina, these genes are known as mod genes. In addition to suppressing vegetative incompatibility, mod mutations cause some developmental defects. This suggests that the molecular mechanisms of vegetative incompatibility and development pathway are interconnected. The mod-E1 mutation was isolated as a suppressor of the developmental defects of the mod-D2 strain. We show here that mod-E1 also partially suppresses vegetative incompatibility, strengthening the link between development and vegetative incompatibility. mod-E1 is the first suppressor of vegetative incompatibility characterized at the molecular level. It encodes a member of the Hsp90 family, suggesting that development and vegetative incompatibility use common steps of a signal transduction pathway. The involvement of mod-E in the sexual cycle has also been further investigated.
摘要
营养体不亲和性在真菌中广泛存在，但其分子机制和生物学功能仍知之甚少。研究营养体不亲和性的一种方法是研究那些其突变能抑制这种现象的基因的功能。在嗜热栖热菌中，这些基因被称为mod基因。除了抑制营养体不亲和性外，mod突变还会导致一些发育缺陷。这表明营养体不亲和性的分子机制与发育途径是相互关联的。mod-E1突变是作为mod-D2菌株发育缺陷的抑制子而分离得到的。我们在此表明，mod-E1也部分抑制营养体不亲和性，强化了发育与营养体不亲和性之间的联系。mod-E1是第一个在分子水平上被表征的营养体不亲和性抑制子。它编码Hsp90家族的一个成员，这表明发育和营养体不亲和性利用信号转导途径的共同步骤。mod-E在有性生殖周期中的作用也已得到进一步研究。
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