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谷胱甘肽S-转移酶编码基因作为能够降解多环芳烃的环境细菌分离株的潜在探针。

Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons.

作者信息

Lloyd-Jones G, Lau P C

机构信息

Manaaki Whenua-Landcare Research Ltd., Hamilton, New Zealand.

出版信息

Appl Environ Microbiol. 1997 Aug;63(8):3286-90. doi: 10.1128/aem.63.8.3286-3290.1997.

DOI:10.1128/aem.63.8.3286-3290.1997
PMID:9251217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC168628/
Abstract

Homologs of the glutathione S-transferase (GST)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading Sphingomonas spp. isolated from New Zealand, Antarctica, and the United States by using PCR primers designed from the GST-encoding gene of Sphingomonas paucimobilis EPA505. Sequence analysis of PCR fragments generated from these isolates and of the GST gene amplified from DNA extracted from polycyclic aromatic hydrocarbon (PAH)-contaminated soil revealed a high degree of conservation, which may make the GST-encoding gene a potentially useful marker for PAH-degrading bacteria.

摘要

通过使用根据少动鞘氨醇单胞菌EPA505的谷胱甘肽S-转移酶（GST）编码基因设计的PCR引物，在从新西兰、南极洲和美国分离出的一组降解芳烃的鞘氨醇单胞菌中鉴定出了GST编码基因的同源物。对这些分离株产生的PCR片段以及从多环芳烃（PAH）污染土壤中提取的DNA扩增出的GST基因进行序列分析，结果显示出高度的保守性，这可能使GST编码基因成为PAH降解细菌的一个潜在有用的标记。

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本文引用的文献

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Glutathione-s-transferase activity and metabolism of glutathione conjugates by rhizosphere bacteria.根际细菌的谷胱甘肽转移酶活性和谷胱甘肽缀合物的代谢。
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Exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione S-transferase superfamily.四氯对苯二酚脱卤酶与谷胱甘肽S-转移酶超家族之间关系的探究。
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Homology between genes for aromatic hydrocarbon degradation in surface and deep-subsurface Sphingomonas strains.表层和深层地下鞘氨醇单胞菌菌株中芳烃降解基因之间的同源性。
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The genus Sphingomonas: physiology and ecology.鞘氨醇单胞菌属：生理学与生态学
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The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance.谷胱甘肽S-转移酶超基因家族：谷胱甘肽S-转移酶的调控及其同工酶在癌症化学保护和耐药性中的作用。
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