Johnsson M, Richardson C F, Bergey E J, Levine M J, Nancollas G H
Department of Chemistry, State University of New York, Buffalo 14214.
Arch Oral Biol. 1991;36(9):631-6. doi: 10.1016/0003-9969(91)90014-l.
The adsorption, at hydroxyapatite surfaces of neutral cystatin SN, acidic cystatin S and the phosphoserine-containing acidic cystatin S1 was compared to that of statherin. The effects of these adsorbed proteins on the constant-composition growth kinetics of hydroxyapatite were also studied. The neutral cystatin SN had a higher adsorption maximum than the acidic cystatins S and S1. Although the affinity of cystatin for hydroxyapatite surfaces was lower than that of statherin, their influence on the growth kinetics of hydroxyapatite was considerably greater, with the acidic cystatin S1 being the most active. At a surface concentration of 7.0 x 10(-8) mol m-2 hydroxyapatite, the cystatins decreased the rate of crystal growth by 80-95% as compared to that in the absence of protein. At this concentration, statherin showed a growth inhibition of 40%.
将中性半胱氨酸蛋白酶抑制剂SN、酸性半胱氨酸蛋白酶抑制剂S和含磷酸丝氨酸的酸性半胱氨酸蛋白酶抑制剂S1在羟基磷灰石表面的吸附与富含组氨酸的磷蛋白进行了比较。还研究了这些吸附蛋白对羟基磷灰石恒定组成生长动力学的影响。中性半胱氨酸蛋白酶抑制剂SN的最大吸附量高于酸性半胱氨酸蛋白酶抑制剂S和S1。虽然半胱氨酸蛋白酶抑制剂对羟基磷灰石表面的亲和力低于富含组氨酸的磷蛋白，但其对羟基磷灰石生长动力学的影响要大得多，其中酸性半胱氨酸蛋白酶抑制剂S1活性最高。在羟基磷灰石表面浓度为7.0×10⁻⁸ mol m⁻²时，与不存在蛋白质时相比，半胱氨酸蛋白酶抑制剂使晶体生长速率降低了80 - 95%。在此浓度下，富含组氨酸的磷蛋白显示出40%的生长抑制率。
