Pavinatto Felippe J, Pavinatto Adriana, Caseli Luciano, Santos David S Dos, Nobre Thatyane M, Zaniquelli Maria E D, Oliveira Osvaldo N
Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil.
Biomacromolecules. 2007 May;8(5):1633-40. doi: 10.1021/bm0701550. Epub 2007 Apr 10.
In this paper we employed phospholipid Langmuir monolayers as membrane models to probe interactions with chitosan. Using a combination of surface pressure--area and surface potential--area isotherms and rheological measurements with the pendent drop technique, we observed that chitosan interacts with phospholipid molecules at the air-water interface. We propose a model in which chitosan interacts with the phospholipids mainly through electrostatic interactions, but also including H-bonding and hydrophobic forces, depending on the phospholipid packing density. At large areas per molecule, chitosan in the subphase adsorbs onto the monolayer, expanding it. At small areas per molecule, chitosan is located in the subsurface. Indeed, a mixed chitosan-phospholipid monolayer can be transferred onto solid supports, even at high surface pressures. The effects of chitosan on the viscoelastic properties of phospholipid monolayers may be taken as evidence for the ability of chitosan to disrupt cell membranes.
在本文中，我们使用磷脂朗缪尔单层膜作为膜模型来探究其与壳聚糖的相互作用。通过结合表面压力-面积和表面电势-面积等温线以及采用悬滴技术进行流变学测量，我们观察到壳聚糖在气-水界面与磷脂分子发生相互作用。我们提出了一个模型，其中壳聚糖与磷脂的相互作用主要通过静电相互作用，但也包括氢键和疏水作用力，这取决于磷脂的堆积密度。在每个分子占据较大面积时，亚相中的壳聚糖吸附到单层膜上，使其膨胀。在每个分子占据较小面积时，壳聚糖位于次表面。实际上，即使在高表面压力下，壳聚糖-磷脂混合单层膜也可以转移到固体支持物上。壳聚糖对磷脂单层膜粘弹性性质的影响可作为壳聚糖破坏细胞膜能力的证据。
