Hathout Yetrib
Children's National Medical Center, Center for Genetic Medicine, Washington, DC 20010, USA.
Expert Rev Proteomics. 2007 Apr;4(2):239-48. doi: 10.1586/14789450.4.2.239.
The secretome, or secretomics, has recently emerged as a new term to describe the global study of proteins that are secreted by a cell, tissue or organism at any given time or under certain conditions. The secretome constitutes an important class of proteins that control and regulate a multitude of biological and physiological processes, thus making it a clinically relevant source for biomarkers and therapeutic target discoveries. There are several approaches that are being implemented to study such a class of proteins; however, each of these approaches has its advantages and limitations. While genome-wide studies using signal predictions can provide a comprehensive analysis of the secretome, the detection and quantification of the actual secreted proteins in a tissue would be more relevant. The goal of this review is to provide an overview of the methods currently used to analyze such a class of proteins, as well as the challenges encountered during the study of the secretome. The implication of studying the cell secretome together with its clinical relevance will be also covered.
分泌蛋白质组学，或分泌蛋白质组学，最近已成为一个新术语，用于描述对细胞、组织或生物体在任何给定时间或特定条件下分泌的蛋白质进行的全面研究。分泌蛋白质组构成了一类重要的蛋白质，它们控制和调节众多生物和生理过程，因此使其成为生物标志物和治疗靶点发现的临床相关来源。目前正在采用几种方法来研究这类蛋白质；然而，这些方法中的每一种都有其优点和局限性。虽然使用信号预测的全基因组研究可以对分泌蛋白质组进行全面分析，但对组织中实际分泌的蛋白质进行检测和定量将更具相关性。本综述的目的是概述目前用于分析这类蛋白质的方法，以及在分泌蛋白质组研究过程中遇到的挑战。同时也将涵盖研究细胞分泌蛋白质组的意义及其临床相关性。
