Omics Sciences, Genomic Sciences, GlaxoSmithKline, Heidelberg, Germany; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
Omics Sciences, Genomic Sciences, GlaxoSmithKline, Heidelberg, Germany.
Mol Cell Proteomics. 2023 Sep;22(9):100636. doi: 10.1016/j.mcpro.2023.100636. Epub 2023 Aug 18.
The active release of proteins into the extracellular space and the proteolytic cleavage of cell surface proteins are key processes that coordinate and fine-tune a multitude of physiological functions. The entirety of proteins that fulfill these extracellular tasks are referred to as the secretome and are of special interest for the investigation of biomarkers of disease states and physiological processes related to cell-cell communication. LC-MS-based proteomics approaches are a valuable tool for the comprehensive and unbiased characterization of this important subproteome. This review discusses procedures, opportunities, and limitations of mass spectrometry-based secretomics to better understand and navigate the complex analytical landscape for studying protein secretion in biomedical science.
蛋白质向细胞外空间的主动释放和细胞表面蛋白的蛋白水解切割是协调和微调多种生理功能的关键过程。完成这些细胞外任务的所有蛋白质被称为分泌组，对于研究与细胞间通讯相关的疾病状态和生理过程的生物标志物具有特殊的意义。基于 LC-MS 的蛋白质组学方法是全面和无偏地描述这个重要亚蛋白组的有价值的工具。本文综述了基于质谱的分泌组学的程序、机会和局限性，以更好地理解和驾驭生物医学科学中研究蛋白质分泌的复杂分析领域。
