Vasilopulos Robert J, Rickard Lora G, Mackin Andrew J, Pharr G Todd, Huston Carla L
Department of Clinical Science, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
J Vet Intern Med. 2007 Mar-Apr;21(2):352-5. doi: 10.1892/0891-6640(2007)21[352:gaogdi]2.0.co;2.
Giardia duodenalis is an intestinal flagellated protozoan that affects many mammalian species often causing severe diarrheal disease. Several different genotypes have been identified (Assemblages A-G). Most isolates recovered from domestic cats have been assigned to either Assemblage A, the zoonotic form of the parasite, or Assemblage F, identified thus far only in cats. Genotypic variation within G. duodenalis may influence clinical presentation and course of disease. Therefore, host-adapted genotypes may not be responsible for diarrheal disease (eg, Assemblage F in cats).
Multiple Giardia genotypes will be present in domestic cats, including Assemblage F, which will not be correlated with clinical signs.
250 domestic cats from eastern Mississippi and northwestern Alabama.
Prevalence survey. Fecal samples evaluated for cysts using a centrifugation concentration technique and a commercially available direct immunoflourescent antibody kit. Giardia isolates were characterized by PCR amplification and sequencing of the glutamate dehydrogenase gene.
Both Assemblage A-I (6/17) and Assemblage F (11/17) were identified. Although Assemblage was significantly associated with age and housing, no association was detected between Assemblage and a variety of other factors including the presence of gastrointestinal signs (acute vomiting, diarrhea, and constipation).
The presence of diarrhea in domestic cats with Giardia cannot be used as a predictor of the presence of zoonotic genotypes in animals within the study area. Although Assemblage A was associated with age and housing, veterinarians should consider any isolation of Giardia from domestic cats as potentially zoonotic.
十二指肠贾第虫是一种肠道鞭毛虫原生动物，可感染多种哺乳动物，常引起严重腹泻病。已鉴定出几种不同的基因型（A - G群）。从家猫分离出的大多数菌株已被归类为A群，即该寄生虫的人畜共患形式，或F群，迄今为止仅在猫中发现。十二指肠贾第虫的基因型变异可能影响临床表现和病程。因此，宿主适应性基因型可能与腹泻病无关（例如猫中的F群）。
家猫中会存在多种贾第虫基因型，包括F群，且与临床症状无关。
来自密西西比州东部和阿拉巴马州西北部的250只家猫。
患病率调查。使用离心浓缩技术和市售直接免疫荧光抗体试剂盒对粪便样本进行囊肿评估。通过谷氨酸脱氢酶基因的PCR扩增和测序对贾第虫分离株进行鉴定。
鉴定出了A - I群（6/17）和F群（11/17）。虽然菌群与年龄和饲养环境显著相关，但未检测到菌群与包括胃肠道症状（急性呕吐、腹泻和便秘）在内的多种其他因素之间存在关联。
在研究区域内，家猫感染贾第虫时出现腹泻不能作为动物存在人畜共患基因型的预测指标。虽然A群与年龄和饲养环境有关，但兽医应将从家猫中分离出的任何贾第虫都视为潜在的人畜共患病原体。
