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松岭印第安人保留地猫狗身上特定人畜共患病原体和媒介传播病原体的流行情况。

Prevalence of Selected Zoonotic and Vector-Borne Agents in Dogs and Cats on the Pine Ridge Reservation.

作者信息

Scorza A Valeria, Lappin Michael R

机构信息

Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

出版信息

Vet Sci. 2017 Sep 4;4(3):43. doi: 10.3390/vetsci4030043.

DOI:10.3390/vetsci4030043
PMID:29056701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5644658/
Abstract

The prevalence of intestinal parasites and vector-borne agents of dogs and cats in the Pine Ridge Reservation, South Dakota were determined. Fecal samples (84 dogs, 9 cats) were examined by centrifugal floatation and by immunofluorescence assay (FA) for and . PCR was performed on [beta-giardin (bg), triose phosphate isomerase (tpi), glutamate dehydrogenase genes (gdh)] and [heat shock protein-70 gene (hsp)] FA positive samples. Cat sera ( = 32) were tested for antibodies against spp., , and FIV, and antigens of FeLV and . Dog sera ( = 82) were tested for antibodies against , , , and and antigen. Blood samples (92 dogs, 39 cats) were assessed by PCR for amplification of DNA of spp., spp., spp., haemoplasmas, and spp. (dogs only). The most significant results were spp. (32% by FA), spp. (17.8%) and spp. (7.1%). The isolates typed as the dog-specific assemblages C or D and four isolates typed as . Antibodies against were detected in 15% of the dogs. Antibodies against spp. and against were detected in 37.5% and 6% of the cats respectively. FeLV antigen was detected in 10% of the cats.

摘要

确定了南达科他州派恩岭印第安人居留地犬猫肠道寄生虫和媒介传播病原体的流行情况。对84份犬粪便样本和9份猫粪便样本进行了离心漂浮法检测，并通过免疫荧光法（FA）检测了[此处原文缺失具体检测项目]。对FA呈阳性的样本进行了[β-贾第虫（bg）、磷酸丙糖异构酶（tpi）、谷氨酸脱氢酶基因（gdh）]和[热休克蛋白-70基因（hsp）]的PCR检测。检测了32份猫血清中针对[此处原文缺失具体病原体]、[此处原文缺失具体病原体]和猫免疫缺陷病毒（FIV）的抗体，以及猫白血病病毒（FeLV）和[此处原文缺失具体病原体]的抗原。检测了82份犬血清中针对[此处原文缺失具体病原体]、[此处原文缺失具体病原体]、[此处原文缺失具体病原体]和[此处原文缺失具体病原体]的抗体以及[此处原文缺失具体病原体]抗原。对92份犬血液样本和39份猫血液样本进行了PCR检测，以扩增[此处原文缺失具体病原体]属、[此处原文缺失具体病原体]属、[此处原文缺失具体病原体]属、血支原体和[此处原文缺失具体病原体]属（仅针对犬）的DNA。最显著的结果是[此处原文缺失具体病原体]属（FA检测阳性率为32%）、[此处原文缺失具体病原体]属（17.8%）和[此处原文缺失具体病原体]属（7.1%）。[此处原文缺失具体病原体]分离株被鉴定为犬特异性组合C或D，4株[此处原文缺失具体病原体]分离株被鉴定为[此处原文缺失具体类型]。15%的犬检测到针对[此处原文缺失具体病原体]的抗体。分别在37.5%和6%的猫中检测到针对[此处原文缺失具体病原体]属和[此处原文缺失具体病原体]的抗体。10%的猫检测到FeLV抗原。

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