Production of glycosaminoglycans by human skin fibroblasts growing in a collagen lattice.

Production of glycosaminoglycans (GAG) by human skin fibroblasts cultured in collagen lattices closely resembled that already described for the same cells grown as monolayers on plastic. There was no inhibition of GAG corresponding to that of DNA and protein synthesis and similar controls by cell density and anti-inflammatory drugs operated in 3-dimensional as in 2-dimensional culture. The bulk of the GAG synthesised in lattice culture was hyaluronic acid, as in monolayers.