用于基因定位的主要斑马鱼多态性资源。

Bradley Kevin M, Elmore J Bradford, Breyer Joan P, Yaspan Brian L, Jessen Jason R, Knapik Ela W, Smith Jeffrey R

Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-0275, USA.

Genome Biol. 2007;8(4):R55. doi: 10.1186/gb-2007-8-4-r55.

We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.

我们在斑马鱼中鉴定出645,088个候选多态性位点，并观察到单核苷酸多态性（SNP）验证率为71%至86%，随着多态性置信度评分的提高而改善。变异位点并非随机分布，存在过量特定的富含T和A的新型基序。我们将一半的多态性位点定位到斑马鱼遗传图谱和物理图谱上，作为定位克隆的资源。我们进一步证明，使用锚定的SNP进行混合分离分析是斑马鱼高通量遗传图谱构建的一种方法。