He Cheng-Qiang, Ding Nai-Zheng, Chen Jian-Guo, Li Yun-Long
College of Life Science, Shandong Normal University, Jinan 250014, China.
Virus Res. 2007 Jun;126(1-2):179-85. doi: 10.1016/j.virusres.2007.02.019. Epub 2007 Apr 10.
Classical swine fever (CSF) virus, one member of the family Flaviviridae is the pathogen of CSF, an economically important and highly contagious disease of pigs. Although homologous recombination has been demonstrated in many other members of the family, it is unknown whether there is recombination in natural populations of CSFV. To detect possible recombination events, we performed a phylogenetic analysis of 25 full-length CSFV strains isolated all over the world. Putative recombinant sequences were identified with the use of SimPlot program. Recombination events were confirmed by bootscaning. A mosaic virus, CSFV 39 (AF407339) isolated in China was found. And its two putative parental-like strains CSFV Shimen (AF333000) and GXWZ02 (AY367767) were identified. Our work revealed that homologous recombination occurred in natural CSFV populations, generating genetic diversity. This would provide some insights for the role homologous recombinant plays in CSFV evolution.
经典猪瘟（CSF）病毒是黄病毒科的成员之一，是猪瘟的病原体，猪瘟是一种对经济有重要影响且极具传染性的猪病。尽管在该科的许多其他成员中已证明存在同源重组，但CSFV自然种群中是否存在重组尚不清楚。为了检测可能的重组事件，我们对从世界各地分离出的25株CSFV全长毒株进行了系统发育分析。使用SimPlot程序鉴定了推定的重组序列。通过bootscaning确认了重组事件。发现了一种在中国分离的嵌合病毒CSFV 39（AF407339）。并鉴定出其两个推定的亲本样毒株CSFV石门株（AF333000）和GXWZ02（AY367767）。我们的研究表明，同源重组发生在CSFV自然种群中，产生了遗传多样性。这将为同源重组在CSFV进化中的作用提供一些见解。
