Hellweg Christine E, Baumstark-Khan Christa
Radiation Biology, Institute of Aerospace Medicine, DLR, Linder Höhe, 51147, Köln, Germany.
Radiat Environ Biophys. 2007 Aug;46(3):269-79. doi: 10.1007/s00411-007-0104-5. Epub 2007 Apr 12.
The cellular protection reaction known as ultraviolet (UV) response leads to increased transcription of several genes. Parts of this transcriptional response are transmitted via activation of the Nuclear factor kappaB (NF-kappaB). The contribution of different UV radiation qualities to this process is not yet known. In a previous work, a stably transfected human cell line was developed which indicates activation of the NF-kappaB pathway by fluorescence of the reporters Enhanced Green Fluorescent Protein (EGFP) and its destabilized variant (d2EGFP) thereby allowing a fast and reliable monitoring of UV effects on the NF-kappaB pathway. Cells were exposed to a mercury low-pressure lamp or to simulated sunlight of different wavelength ranges and subjected to flow cytometric analysis after different post-irradiation periods. Growth capacity of cells after UV irradiation was quantified using a luminance measurement of crystal violet stained cell layers. In contrast to UVC and UVB, UVA radiation induced d2EGFP expression and NF-kappaB activation in a non-cytotoxic dose range. These results show that NF-kappaB plays a role in the UVA-induced gene activation in a non-cytotoxic dose range in a human epithelial cell line.
被称为紫外线（UV）反应的细胞保护反应会导致多个基因的转录增加。这种转录反应的部分过程是通过核因子κB（NF-κB）的激活来传递的。不同紫外线辐射特性对这一过程的作用尚不清楚。在之前的一项工作中，开发了一种稳定转染的人类细胞系，该细胞系通过报告基因增强型绿色荧光蛋白（EGFP）及其不稳定变体（d2EGFP）的荧光来指示NF-κB途径的激活，从而能够快速可靠地监测紫外线对NF-κB途径的影响。将细胞暴露于汞低压灯或不同波长范围的模拟阳光下，并在不同的照射后时间段进行流式细胞术分析。使用结晶紫染色细胞层的亮度测量来量化紫外线照射后细胞的生长能力。与UVC和UVB不同，UVA辐射在非细胞毒性剂量范围内诱导d2EGFP表达和NF-κB激活。这些结果表明，在人类上皮细胞系中，NF-κB在非细胞毒性剂量范围内的UVA诱导的基因激活中发挥作用。
