Kung L, Schmidt R J, Ebling T E, Hu W
Department of Animal and Food Sciences, University of Delaware, Newark 19716-2150, USA.
J Dairy Sci. 2007 May;90(5):2309-14. doi: 10.3168/jds.2006-713.
Experiments were conducted to evaluate the effects of inoculating high-moisture corn (HMC) with Lactobacillus buchneri 40788 on silage fermentation and aerobic stability. In the first experiment, HMC (73% DM) was ground and treated with nothing, L. buchneri 40788 to achieve 6.6 x 10(5) cfu/g of HMC (LB), a mixture of enzymes (ENZ), LB + ENZ, or 0.1% (wet weight basis) of a liquid mold inhibitor and was ensiled in 20-L bucket silos for 90 d. Treatments with LB and LB + ENZ increased the concentrations of acetic acid and improved the aerobic stability of ground HMC relative to other treatments. Treatment ENZ had no effect on the chemical composition or aerobic stability of ground HMC. The only effect of the liquid mold inhibitor relative to untreated HMC was that it increased the concentration of propionic acid, but this did not improve its aerobic stability. In a second experiment, HMC (75% DM) was harvested as the intact, whole grain and treated with nothing, L. buchneri 40788 to achieve 4 x 10(5) cfu/g of HMC, L. buchneri 40788 to achieve 6 x 10(5) cfu/g of HMC, or L. buchneri 40788 to achieve 8 x 10(5) cfu/g of HMC and ensiled for 120 d. Treatments with L. buchneri 40788 resulted in whole HMC with lower concentrations of water-soluble carbohydrates; higher concentrations of lactic, acetic, and propionic acids; and greater numbers of lactic acid bacteria but fewer molds when compared with untreated corn. As a group, inoculated silages were more aerobically stable than untreated silage, but increasing levels of application did not further improve the response. These experiments showed that addition of L. buchneri 40788, but not addition of an enzyme mixture or a liquid mold inhibitor, improved the aerobic stability of ground and whole HMC harvested between 73 and 75% DM.
开展了试验以评估用布氏乳杆菌40788接种高水分玉米（HMC）对青贮发酵及好氧稳定性的影响。在第一个试验中，将HMC（干物质含量73%）粉碎，分别不进行处理、用布氏乳杆菌40788处理使其在HMC中达到6.6×10⁵ cfu/g（LB）、用酶混合物（ENZ）处理、用LB + ENZ处理，或用0.1%（湿重计）的液体防霉剂处理，然后装入20升的桶式青贮窖中青贮90天。与其他处理相比，用LB和LB + ENZ处理提高了乙酸浓度并改善了粉碎后HMC的好氧稳定性。ENZ处理对粉碎后HMC的化学成分或好氧稳定性没有影响。与未处理的HMC相比，液体防霉剂的唯一作用是提高了丙酸浓度，但这并未改善其好氧稳定性。在第二个试验中，收获干物质含量75%的完整全粒HMC，分别不进行处理、用布氏乳杆菌40788处理使其在HMC中达到4×10⁵ cfu/g、用布氏乳杆菌40788处理使其在HMC中达到6×10⁵ cfu/g，或用布氏乳杆菌40788处理使其在HMC中达到8×10⁵ cfu/g，然后青贮120天。与未处理的玉米相比，用布氏乳杆菌40788处理的全粒HMC水溶性碳水化合物浓度较低；乳酸、乙酸和丙酸浓度较高；乳酸菌数量较多但霉菌数量较少。作为一个整体，接种的青贮料比未处理的青贮料好氧稳定性更高，但增加施用水平并未进一步改善效果。这些试验表明，添加布氏乳杆菌40788可改善干物质含量在73%至75%之间收获的粉碎及完整全粒HMC的好氧稳定性，而添加酶混合物或液体防霉剂则无此效果。
