Herrmann J E, Cliver D O
Appl Microbiol. 1968 Oct;16(10):1564-9. doi: 10.1128/am.16.10.1564-1569.1968.
A method previously reported for detecting virus in a model system composed of cottage cheese contaminated with coxsackievirus type A9 has been adapted to detecting selected strains of enteroviruses in a variety of foods. Bentonite is omitted and serum is added for extracting virus from low-protein foods. Samples of foods, usually 25 g, must contain at least 3 to 4 plaque-forming units for a 50% probability of detecting virus. Sensitivity in detecting echovirus type 6 was lower than that for the other viruses used. After extraction from potato salad, poliovirus type 2 was completely reactivated if it had been neutralized with coproantibody, but it was only partially reactivated if neutralized with hyperimmune rabbit serum.
一种先前报道的用于在由被A9型柯萨奇病毒污染的酪乳组成的模型系统中检测病毒的方法，已被改编用于检测多种食品中选定的肠道病毒毒株。省略了膨润土，并添加血清以从低蛋白食品中提取病毒。食品样本通常为25克，要以50%的概率检测到病毒，必须至少含有3至4个空斑形成单位。检测6型艾柯病毒的灵敏度低于所使用的其他病毒。从土豆沙拉中提取后，2型脊髓灰质炎病毒如果已用粪便抗体中和则会完全重新激活，但如果用超免疫兔血清中和则只会部分重新激活。
