Dutra-Correa Maristela, Anauate-Netto Camillo, Arana-Chavez Victor E
Department of Operative Dentistry, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, SP, Brazil.
Arch Oral Biol. 2007 Sep;52(9):850-5. doi: 10.1016/j.archoralbio.2007.03.003. Epub 2007 Apr 12.
Bovine teeth have been widely used in studies focusing adhesion to dentine over the last years. However, little is known about main structural aspects of bovine dentine, especially regarding density and diameter of its tubules. Thirty bovine incisors were randomly divided into two groups. In group I, teeth were cross-sectioned at three depths: outer, middle and inner. The dentinal surfaces were etched with 35% phosphoric acid for 90s. In group II, the teeth were fractured at the same three depths. All the specimens were processed and examined in a Jeol 6100 SEM. Fifteen micrographs from each depth were obtained for the two groups at a magnification of 2500x. To determine the diameter of the dentinal tubules, the LEICA Q500 MC software was used. All the results were statistically processed in the EXCEL software. The dentinal tubules were wider at the outer regions (GI: 5.21+/-0.64microm; GII: 2.30+/-0.09microm) than that at the inner regions (GI: 2.71+/-0.72microm; GII: 1.77+/-0.06microm). Tubular density was higher at the inner regions (50310+/-11178tubules/mm(2)) than that at the outer regions (18772+/-2951tubules/mm(2)). In addition, the peritubular dentine was wider at the outer depth than at the inner one. Our results showed that bovine dentine possesses some structural aspects different from those previously reported for human dentine, mainly related to the diameter of dentinal tubules and to the thickness of peritubular dentine at the several depths.
在过去几年中，牛牙已广泛用于专注于牙本质黏附的研究。然而，对于牛牙本质的主要结构方面，尤其是其小管的密度和直径，人们了解甚少。30颗牛切牙被随机分为两组。在第一组中，牙齿在三个深度处进行横截面切割：外层、中层和内层。牙本质表面用35%的磷酸蚀刻90秒。在第二组中，牙齿在相同的三个深度处折断。所有标本均在Jeol 6100扫描电子显微镜下进行处理和检查。两组在每个深度处均获得15张放大倍数为2500倍的显微照片。为了确定牙本质小管的直径，使用了徕卡Q500 MC软件。所有结果均在EXCEL软件中进行统计处理。牙本质小管在外层区域（第一组：5.21±0.64微米；第二组：2.30±0.09微米）比在内层区域（第一组：2.71±0.72微米；第二组：1.77±0.06微米）更宽。小管密度在内层区域（50310±11178个小管/mm²）比外层区域（18772±2951个小管/mm²）更高。此外，管周牙本质在外层深度比内层更宽。我们的结果表明，牛牙本质具有一些与先前报道的人牙本质不同的结构方面，主要与牙本质小管的直径以及不同深度处管周牙本质的厚度有关。
