Ng S C, Bongso A, Ratnam S S
Department of Obstetrics and Gynaecology, National University of Singapore.
Fertil Steril. 1991 Dec;56(6):1117-23. doi: 10.1016/s0015-0282(16)54726-8.
To determine outcome after microinjection with very poor quality sperm and after failed fertilization.
Group 1, fresh oocytes from patients with very low sperm density and motility on the day of oocyte recovery; Group 2, 1-day-old oocytes that failed to fertilize.
All material was obtained from the National University Hospital.
One hundred and thirty-one from group 1; 35 from group 2.
Sperm was injected subzonally or directly into the ooplasm.
Normal and abnormal fertilization and pregnancy.
Subzonal transfer was done on 771 oocytes in group 1 and 188 oocytes in group 2. Multiple sperm were transferred [mean of 6.6 for group 1 and 7.3 for group 2]. Monospermic fertilization occurred in 16.6% and 14.9%, respectively. Polyspermy and parthenogenetic activation were low at 2.3% and 2.8%, respectively. Five pregnancies were obtained, but only one delivered. Ooplasmic injection (single sperm heads) was done in 38 oocytes from three patients with extremely severe oligozoospermia; only four two-pronuclear zygotes were obtained and replaced into two patients, without any resulting pregnancy.
Subzonal transfer may be a viable technique for patients with severe sperm problems.
确定使用质量极差的精子进行显微注射以及受精失败后的结果。
第1组，在卵母细胞采集当天，采集精子密度和活力极低的患者的新鲜卵母细胞；第2组，未受精的1日龄卵母细胞。
所有材料均取自国立大学医院。
第1组131例；第2组35例。
将精子注入透明带下或直接注入卵质内。
正常受精与异常受精及妊娠情况。
第1组对771个卵母细胞、第2组对188个卵母细胞进行了透明带下移植。移植了多个精子（第1组平均为6.6个，第2组平均为7.3个）。单精受精分别为16.6%和14.9%。多精受精和孤雌激活率较低，分别为2.3%和2.8%。获得了5例妊娠，但仅1例分娩。对3例极重度少精子症患者的38个卵母细胞进行了卵质内注射（单个精子头部）；仅获得4个双原核受精卵，并移植到2例患者体内，但未产生任何妊娠。
透明带下移植对于存在严重精子问题的患者可能是一种可行的技术。
