Fishel S, Timson J, Lisi F, Rinaldi L
University Hospital, Queen's Medical Centre, Nottingham, United Kingdom.
Fertil Steril. 1992 Apr;57(4):840-9. doi: 10.1016/s0015-0282(16)54968-1.
To evaluate subzonal insemination for the procurement of fertilization in infertile males.
The spermatozoa of infertile males was used, where possible, for in vitro fertilization (IVF) and subzonal insemination with the same cohort of oocytes obtained from their partner.
An individual unit in a general hospital, Clinical Nomentana, Rome, Italy.
Males who had repeatedly failed to achieve conception in vitro by IVF or in whom seminal parameters were unacceptable for IVF were treated.
Two hundred twenty-five transvaginal oocyte retrievals were performed.
The incidence of fertilization, cleavage, and pregnancy was evaluated in relation to the numbers of spermatozoa injected and the individual spermatozoa parameters.
Fertilization occurred in 39% of patients and 16% of eggs, and 12 clinical pregnancies were established. Fertilization increased with increasing numbers of spermatozoa injected. Fertilization with subzonal insemination was significantly greater (39%) than after in vitro insemination (6%) (P less than 0.0001).
Subzonal insemination improved the incidence of fertilization in this series of patients, and a new classification of spermatozoa based on total motile count aided prognosis for fertilization.
评估卵周隙内授精在不育男性中实现受精的效果。
尽可能使用不育男性的精子，与从其伴侣获取的同一批卵母细胞进行体外受精（IVF）和卵周隙内授精。
意大利罗马诺门塔纳临床综合医院的一个独立科室。
接受治疗的男性为多次通过IVF未能在体外受孕者或精液参数不符合IVF要求者。
进行了225次经阴道取卵操作。
根据注射精子数量和单个精子参数评估受精、卵裂和妊娠发生率。
39%的患者和16%的卵子发生受精，确立了12例临床妊娠。受精率随注射精子数量增加而提高。卵周隙内授精的受精率（39%）显著高于体外授精（6%）（P<0.0001）。
卵周隙内授精提高了该系列患者的受精率，基于总活动精子数的精子新分类有助于受精预后评估。
