Myllyluoma E, Kajander K, Mikkola H, Kyrönpalo S, Rasmussen M, Kankuri E, Sipponen P, Vapaatalo H, Korpela R
Institute of Biomedicine, Pharmacology, University of Helsinki, Finland.
Dig Liver Dis. 2007 Jun;39(6):516-23. doi: 10.1016/j.dld.2007.02.015. Epub 2007 Apr 11.
Previously we showed that a probiotic combination with L. rhamnosus GG was beneficial as an adjuvant therapy during H. pylori eradication.
To evaluate whether probiotic combination with LGG adheres to the upper gastrointestinal mucosa and modifies H. pylori colonisation and H. pylori induced inflammation.
Thirteen patients referred for gastroduodenoscopy received a drink consisting of equal doses (2.5x10(9)CFU) of LGG, L. rhamnosus LC705, Propionibacterium freudenreichii JS and Bifidobacterium lactis Bb12 daily. Recovery of probiotics in biopsies (antrum, corpus, duodenum) and faecal samples was evaluated by strain-specific quantitative polymerase chain reaction. H. pylori colonization and gastric inflammation was investigated by urease activity ((13)C-urea breath test), histology and serum pepsinogen I, II and gastrin-17 measurements.
Twelve patients were fully investigated; of these three of the patients had LGG adhering to the biopsies at end of the intervention. Other probiotic strains were not detected, even though the recovery of all individual probiotic strains from the faeces was significantly increased (p<0.01). After the treatment, the level of (13)C-urea breath test (p=0.063) and gastrin-17 (p=0.046) decreased.
The decreases in (13)C-urea breath test and gastrin-17 indicate that the probiotic combination exerts a beneficial effect on gastric mucosa in H. pylori infected patients. LGG showed marginal ability to adhere to the upper gastrointestinal tract mucosa.
此前我们发现，含有鼠李糖乳杆菌GG的益生菌组合作为幽门螺杆菌根除治疗期间的辅助疗法有益。
评估含LGG的益生菌组合是否能黏附于上消化道黏膜，并改变幽门螺杆菌定植及幽门螺杆菌诱导的炎症。
13例接受胃十二指肠镜检查的患者每天饮用一份饮品，其中含有等量剂量（2.5×10⁹CFU）的LGG、鼠李糖乳杆菌LC705、费氏丙酸杆菌JS和乳酸双歧杆菌Bb12。通过菌株特异性定量聚合酶链反应评估活检组织（胃窦、胃体、十二指肠）和粪便样本中益生菌的恢复情况。通过尿素酶活性（¹³C-尿素呼气试验）、组织学检查以及血清胃蛋白酶原I、II和胃泌素-17测量来研究幽门螺杆菌定植和胃部炎症。
12例患者完成了全面检查；其中3例患者在干预结束时活检组织中有LGG黏附。未检测到其他益生菌菌株，尽管从粪便中回收的所有单个益生菌菌株数量均显著增加（p<0.01）。治疗后，¹³C-尿素呼气试验水平（p=0.063）和胃泌素-17水平（p=0.046）下降。
¹³C-尿素呼气试验和胃泌素-17水平降低表明，该益生菌组合对幽门螺杆菌感染患者的胃黏膜有有益作用。LGG显示出黏附于上消化道黏膜的能力较弱。
