Mulder I E, Wadsworth S, Secombes C J
Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
Fish Shellfish Immunol. 2007 Oct;23(4):747-59. doi: 10.1016/j.fsi.2007.02.002. Epub 2007 Feb 24.
Gene expression of a number of cytokines in the intestine of rainbow trout (Oncorhynchus mykiss) was investigated after challenge with a pathogenic strain of Aeromonas salmonicida. Fish were exposed to A. salmonicida by immersion in a bacterial suspension (bath challenge) and tissue samples of the distal and proximal intestine were collected at days 0, 2, 4, 6 and 8 post-exposure. Head kidney tissue was also collected to assess the effect in a systemic immune tissue. A classic profile of pro-inflammatory cytokine upregulation was observed in the proximal intestine of fish infected by bath challenge, as determined by semi-quantitative RT-PCR. Expression of IL-1beta, IL-8, TNF-alpha and IFN-gamma was increased in the proximal intestine. TGF-beta was significantly decreased in the distal intestine. In the head kidney, infection with A. salmonicida by bath challenge caused decreased expression levels of IL-1beta, IL-8, TNF-alpha and TGF-beta. The results are discussed in the context of potential immune mechanisms in the gut to prevent infection.
在用杀鲑气单胞菌致病菌株攻击后，研究了虹鳟（Oncorhynchus mykiss）肠道中多种细胞因子的基因表达。通过将鱼浸入细菌悬液（浸浴攻击）使其暴露于杀鲑气单胞菌，并在暴露后第0、2、4、6和8天收集远端和近端肠道的组织样本。还收集了头肾组织以评估对全身免疫组织的影响。通过半定量RT-PCR测定，在浸浴攻击感染的鱼的近端肠道中观察到促炎细胞因子上调的典型特征。近端肠道中IL-1β、IL-8、TNF-α和IFN-γ的表达增加。远端肠道中TGF-β显著降低。在头肾中，浸浴攻击感染杀鲑气单胞菌导致IL-1β、IL-8、TNF-α和TGF-β的表达水平降低。在肠道预防感染的潜在免疫机制背景下对结果进行了讨论。
