Fimognari Carmela, Lenzi Monia, Sciuscio Davide, Cantelli-Forti Giorgio, Hrelia Patrizia
Department of Pharmacology, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy.
In Vivo. 2007 Mar-Apr;21(2):377-80.
Sulforaphane (SFN) is an isothiocyanate that is present in widely consumed vegetables. Previous studies have shown that SFN is effective in preventing carcinogenesis induced by carcinogens in rodents. Recently it was found that SFN could also inhibit cell proliferation and induce apoptosis in several tumor cell lines. In the present study, the possible cell-cycle specificity of SFN-mediated apoptosis was investigated.
Cells were synchronized by thymidine block. Analysis of the cell-cycle and apoptosis induction was performed using flow cytometry.
Flow cytometric assessment of the extent of apoptosis in cells synchronized by thymidine block revealed that cells were most sensitive to SFN in the G -phase, less sensitive in the G2/M-phase and least sensitive during the S-phase.
These findings suggest that cell vulnerability to SFN-mediated apoptosis is subject to regulation by cell-cycle-dependent mechanisms.
萝卜硫素（SFN）是一种异硫氰酸盐，存在于广泛食用的蔬菜中。先前的研究表明，SFN在预防啮齿动物致癌物诱导的致癌作用方面是有效的。最近发现，SFN还可以抑制几种肿瘤细胞系中的细胞增殖并诱导凋亡。在本研究中，研究了SFN介导的凋亡可能的细胞周期特异性。
通过胸腺嘧啶核苷阻断使细胞同步化。使用流式细胞术进行细胞周期和凋亡诱导分析。
通过胸腺嘧啶核苷阻断同步化的细胞中凋亡程度的流式细胞术评估显示，细胞在G1期对SFN最敏感，在G2/M期较不敏感，在S期最不敏感。
这些发现表明，细胞对SFN介导的凋亡的易感性受细胞周期依赖性机制的调节。
