Chalabi Nasséra, Delort Laetitia, Satih Samir, Déchelotte Pierre, Bignon Yves-Jean, Bernard-Gallon Dominique J
Département d'Oncogénétique, Centre Jean Perrin, Clermont-Ferrand Cedex 1, France.
J Histochem Cytochem. 2007 Sep;55(9):877-83. doi: 10.1369/jhc.7A7185.2007. Epub 2007 Apr 16.
Lycopene, the major carotenoid found in tomatoes, is a potent antioxidant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RARalpha, RARbeta, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 microM lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RARalpha and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines.
番茄红素是番茄中发现的主要类胡萝卜素，是一种强效抗氧化剂，与预防诸如乳腺癌等退行性疾病有关。这种作用可能归因于番茄红素与视黄酸受体之间的相互作用，以及对间隙连接通讯的刺激和连接蛋白43的合成。在两种乳腺癌细胞系MCF-7和MDA-MB-231以及一种纤维囊性营养不良细胞系MCF-10a中，在暴露于10微摩尔番茄红素48小时后，使用免疫组织化学分析RARalpha、RARbeta和Cx43蛋白的表达。然后进行实时定量PCR分析以测量mRNA表达。在两种乳腺细胞系中，RARalpha和Cx43的表达在mRNA和蛋白质水平上均增加。
