Hong Ji-Young, Nam Joo-Won, Seo Eun-Kyoung, Lee Sang Kook
College of Pharmacy, Ewha Womans University, Seoul 120-750, Korea.
Chem Pharm Bull (Tokyo). 2010 Feb;58(2):234-7. doi: 10.1248/cpb.58.234.
Two new daphnane-type diterpene esters, yuanhuahine (1) and yuanhualine (2), were isolated from the flowers of Daphne genkwa (Thymelaeaceae) along with three known diterpene esters, yuanhuacine (3), yuanhuadine (4), and yuanhuagine (5). Their structures were determined by a combination of 1D and 2D NMR experiments, including correlation spectroscopy (COSY), heteronuclear multiple quantum correlation (HMQC), heteronuclear multiple bond correlation (HMBC), and rotating frame Overhauser enhancement spectroscopy (ROESY) sequences, and mass spectrometry. All the isolated compounds were tested against A549 human lung cancer cells and MRC-5 human normal lung epithelial cells. Compounds 1-5 exhibited potent anti-proliferative effects against A549 lung cancer cells with IC(50) values of 12-53 nM, whereas these compounds were relatively non-cytotoxic against MRC-5 normal lung epithelial cells.
从芫花（瑞香科）的花中分离出两种新的瑞香烷型二萜酯，芫花酯壬（1）和芫花酯甲（2），以及三种已知的二萜酯，芫花酯丙（3）、芫花酯丁（4）和芫花酯戊（5）。通过一维和二维核磁共振实验相结合确定了它们的结构，包括相关光谱法（COSY）、异核多量子相关（HMQC）、异核多键相关（HMBC）和旋转框架奥弗豪泽增强光谱法（ROESY）序列，以及质谱分析。所有分离出的化合物均针对A549人肺癌细胞和MRC-5人正常肺上皮细胞进行了测试。化合物1-5对A549肺癌细胞表现出强大的抗增殖作用，IC(50)值为12-53 nM，而这些化合物对MRC-5正常肺上皮细胞相对无细胞毒性。
