流动相组成对分配色谱法评价亲脂性的影响。
Influence of mobile phase composition on evaluation of lipophilicity by partition chromatography.
作者信息
Kuchar M, Kraus E, Jelínková M
机构信息
Research Institute for Pharmacy and Biochemistry, Prague, Czechoslovakia.
出版信息
J Chromatogr. 1991 Sep 20;557(1-2):399-411. doi: 10.1016/s0021-9673(01)87148-x.
The problems of the concentration dependence of retention indices and the applicability of extrapolated values in the evaluation of lipophilicity were studied. The reversed-phase high-performance liquid chromatography of arylalkanoic acids were carried out with experimental data for substituted estra-1,3,5 (10)-trienes, benzodiazepines, dermorphine derivatives and dansylamides selected from the literature for this purpose. Fair linear relationships between slopes of concentration dependences and extrapolated and non-extrapolated values of RM and log k' were found. Equivalence of these indices in the evaluation of lipophilicity can be inferred. Statistically significant dependences of log P (sigma pi) values on concentration slopes make it possible to use them as new parameters of lipophilicity. The goodness of fit of these relationships increases when the values of ET(30), as a measure of the solvatochromic solvent polarity of mobile phases, are used instead of the change in modifier concentration.
研究了保留指数的浓度依赖性问题以及外推值在亲脂性评估中的适用性。为此，利用从文献中选取的取代雌甾 - 1,3,5（10）- 三烯、苯二氮䓬类、皮吗啡衍生物和丹磺酰胺的实验数据，进行了芳基链烷酸的反相高效液相色谱分析。发现浓度依赖性斜率与RM和log k'的外推值及非外推值之间存在良好的线性关系。由此可以推断这些指数在亲脂性评估中的等效性。log P（σπ）值对浓度斜率具有统计学上显著的依赖性，这使得可以将它们用作亲脂性的新参数。当使用ET(30)值（作为流动相溶剂化显色溶剂极性的度量）代替改性剂浓度的变化时，这些关系的拟合优度会提高。
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