Cowan D A, Kicman A T, Walker C J, Wheeler M J
Drug Control and Teaching Centre, King's College, London.
J Endocrinol. 1991 Oct;131(1):147-54. doi: 10.1677/joe.0.1310147.
Abnormal ratios of testosterone to epitestosterone (T/E) and testosterone to LH (T/LH) in the urine of male athletes are indicative of testosterone administration. The T/E ratio has been adopted by the International Olympic Committee as the sole criterion used in the detection of testosterone administration. An athlete is usually considered to have failed a drug test if the urinary T/E ratio is greater than 6. Human chorionic gonadotrophin (hCG) has been used by some male athletes to stimulate testicular secretion of testosterone. The purpose of this investigation was to examine whether the urinary T/E ratio can remain unaffected by administration of hCG to normal adult males. Administration of hCG resulted in large increases in serum testosterone concentrations and urinary T/LH ratios but small changes in urinary T/E ratios of two subjects (maximum T/E values observed were 0.8 and 1.2 respectively). These observations suggest that the urinary T/LH ratio is a valuable indicator of hCG as well as of testosterone administration. This study is the first to measure urinary T/LH ratios using the technique of gas chromatography-mass spectrometry for quantification of testosterone, and highly specific monoclonal antibodies for the measurement of LH. An ultrafiltration method is proposed as part of a confirmatory procedure to be adopted in the measurement of urinary gonadotrophins for drug control in sport.
男性运动员尿液中睾酮与表睾酮（T/E）以及睾酮与促黄体生成素（T/LH）的比例异常表明使用了睾酮。国际奥委会已采用T/E比值作为检测睾酮使用情况的唯一标准。如果尿液T/E比值大于6，运动员通常会被认定药检不合格。一些男性运动员使用人绒毛膜促性腺激素（hCG）来刺激睾丸分泌睾酮。本研究的目的是检验对正常成年男性施用hCG后尿液T/E比值是否不受影响。施用hCG导致两名受试者的血清睾酮浓度和尿液T/LH比值大幅升高，但尿液T/E比值变化较小（观察到的最大T/E值分别为0.8和1.2）。这些观察结果表明，尿液T/LH比值是hCG以及睾酮使用情况的重要指标。本研究首次采用气相色谱 - 质谱技术定量睾酮以及高特异性单克隆抗体测量LH来测定尿液T/LH比值。提出一种超滤方法作为在体育赛事药物控制中测定尿促性腺激素的确认程序的一部分。
