ART患者卵泡抽吸物中卵巢巨噬细胞的意义
Significance of ovarian macrophages in the follicular aspirates from ART patients.
作者信息
Barañao Rosa Inés, Quintana Ramiro, Martín Alina, Kopcow Laura, Marconi Guillermo, Sueldo Carlos
机构信息
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (National Research Council), Buenos Aires, Argentina.
出版信息
J Assist Reprod Genet. 2007 Apr;24(4):137-42. doi: 10.1007/s10815-006-9102-9. Epub 2007 Feb 16.
PURPOSE
To evaluate the percentages of macrophages present in granulosa cells (GC) cultures from patients with different responses to the hyperstimulation, in relation to the percentages of apoptotic cells (ApC), as well as to the release of cytokines.
METHODS
We studied 42 patients: 12 Hyporesponders, (with < or =4 follicles), 15 Normoresponders, (5-14 follicles), and 15 Hyperresponders, (> or =15 follicles). In GC cultures percentages of macrophages and ApC were counted and, in the conditioned media, cytokines were measured.
RESULTS
Percentages of macrophages were significantly higher in GC cultures from Hyporesponders compared with Hyperresponders patients. Also, the percentages of ApC cells were the highest in Hyporesponders. On the contrary, cytokines concentrations were the lowest in this group.
CONCLUSIONS
The low ovarian response is probably due to the decreased angiogenesis, which in turn produces increased apoptosis and decreased production of cytokines. The increased percentage of macrophages could be related to increased frequency of apoptotic cells.
目的
评估对超促排卵有不同反应的患者颗粒细胞（GC）培养物中巨噬细胞的百分比，以及与凋亡细胞（ApC）百分比和细胞因子释放的关系。
方法
我们研究了42例患者：12例低反应者（卵泡数≤4个），15例正常反应者（卵泡数5 - 14个），以及15例高反应者（卵泡数≥15个）。在GC培养物中计数巨噬细胞和ApC的百分比，并在条件培养基中测量细胞因子。
结果
与高反应者患者相比，低反应者的GC培养物中巨噬细胞百分比显著更高。此外，ApC细胞百分比在低反应者中最高。相反，该组细胞因子浓度最低。
结论
卵巢低反应可能是由于血管生成减少，进而导致凋亡增加和细胞因子产生减少。巨噬细胞百分比增加可能与凋亡细胞频率增加有关。
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