Banér Johan, Gyarmati Péter, Yacoub Alia, Hakhverdyan Mikhayil, Stenberg Johan, Ericsson Olle, Nilsson Mats, Landegren Ulf, Belák Sándor
Joint Research and Development Division, Department of Virology, The National Veterinary Institute and the Swedish University of Agricultural Sciences, Ulls väg 2B, SE-75189 Uppsala, Sweden.
J Virol Methods. 2007 Aug;143(2):200-6. doi: 10.1016/j.jviromet.2007.03.004. Epub 2007 Apr 23.
The World Organization for Animal Health (Office International des Epizooties, OIE) includes the diseases caused by foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), as "Diseases Notifiable to the OIE". Foot-and-mouth disease (FMD) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. Efficient laboratory techniques are therefore required for detection and identification of the viruses causing similar vesicular symptoms in swine. A rapid method is described using padlock probes and microarrays to detect simultaneously and differentiate the three viruses in a single reaction, as well as providing serotype information in cases of VSV infection. The padlock probe/microarray assay detected successfully and identified 39 cDNA samples of different origin representing the three viruses. The results were in complete agreement with identities and serotypes determined previously. This novel virus detection method is discussed in terms of usefulness and further development.
世界动物卫生组织（国际兽疫局，OIE）将口蹄疫病毒（FMDV）、猪水疱病病毒（SVDV）和水疱性口炎病毒（VSV）引起的疾病列为“应向OIE通报的疾病”。口蹄疫（FMD）疫情具有严重的经济和社会影响，且无法根据临床症状与其他两种病毒引起的疾病相区分。因此，需要高效的实验室技术来检测和鉴定引起猪类似水疱症状的病毒。本文描述了一种快速方法，该方法使用锁式探针和微阵列在单一反应中同时检测和区分这三种病毒，并在VSV感染的情况下提供血清型信息。锁式探针/微阵列分析成功检测并鉴定了代表这三种病毒的39个不同来源的cDNA样本。结果与先前确定的身份和血清型完全一致。本文从实用性和进一步发展的角度对这种新型病毒检测方法进行了讨论。
