Yadav Brijesh S, Pokhriyal Mayank, Ratta Barkha, Kumar Ajay, Saxena Meeta, Sharma Bhaskar
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India - 243122.
Bioinformation. 2015 Nov 30;11(11):489-92. doi: 10.6026/97320630011489. eCollection 2015.
Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified Bovine herpes virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2) in clinical samples. Microarray identified specific probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that customized application requires a detailed cost efficiency calculation.
近年来，使用定制微阵列芯片对印度家畜进行病毒诊断的趋势日益增强。因此，有可能设计出针对感染印度家畜的病毒的定制微阵列芯片。定制微阵列芯片在临床样本中鉴定出了牛疱疹病毒1型（BHV-1）、犬腺病毒1型（CAV-1）和犬细小病毒2型（CPV-2）。在所有临床样本和已知样本中，使用逆转录聚合酶链反应（RT-PCR）进一步确认了微阵列鉴定出的特异性探针。因此，在传统方法不适用的情况下，微阵列芯片可应用于印度家畜的病毒性疾病爆发。需要注意的是，定制应用需要进行详细的成本效益计算。
