Graham B J, Bengali Z, Vande Woude G F
J Virol. 1978 Mar;25(3):878-87. doi: 10.1128/JVI.25.3.878-887.1978.
The origin of defective DNA (dDNA) of the Patton strain of herpes simplex virus type 1 (HSV-1) was physically mapped with BamHI in the parental DNA. The dDNA obtained from virus passaged at high multiplicities of infection was resistant to cleavage with HindIII, whereas digestion with EcoRI yielded a cluster of fragments 5.4 to 5.7 megadaltons (Mdal) in size. Cleavage with BamHI gave a cluster of fragments 2.6 to 3.2 Mdal in size, plus two homogeneous, comigrating 1-Mdal fragments. One of the latter fragments contained the single EcoRI site approximately 65 base pairs from one end. Hybridization of in vitro labeled dDNA probe to EcoRI, HindIII, BamHI, and Hpa I digests of nondefective HSV-1 DNA demonstrated that, in addition to the S-region terminal repeat, only one end of the S region was involved in the generation of this class of dDNA. Thus, the dDNA probe did not hybridize to either the S region 3.0-Mdal HindIIIN fragment or a 3.0-Mdal BamHI fragment of the adjacent 8.7-Mdal HindIIIG fragment, but did hybridize to four BamHI fragments of HindIII G (approximately 5.7 Mdal). The cluster of 2.6- to 3.2-Mdal fragments obtained with BamHI digestion of dDNA appears to represent a novel junction between the termination of dDNA adjacent to the 3.0-Mdal BamHI fragment in HindIII G and the 2.0- to 2.3-Mdal BamHI fragment terminal in HSV-1 DNA.
用BamHI对1型单纯疱疹病毒（HSV-1）帕顿株的缺陷DNA（dDNA）起源在亲本DNA中进行了物理定位。从高感染复数传代的病毒中获得的dDNA对HindIII切割具有抗性，而用EcoRI消化则产生一组大小为5.4至5.7兆道尔顿（Mdal）的片段。用BamHI切割产生一组大小为2.6至3.2 Mdal的片段，外加两个均一的、共迁移的1-Mdal片段。后一种片段之一在距一端约65个碱基对处含有单个EcoRI位点。体外标记的dDNA探针与无缺陷HSV-1 DNA的EcoRI、HindIII、BamHI和Hpa I消化产物杂交表明，除了S区域末端重复序列外，只有S区域的一端参与了这类dDNA的产生。因此，dDNA探针不与S区域3.0-Mdal HindIIIN片段或相邻8.7-Mdal HindIIIG片段的3.0-Mdal BamHI片段杂交，但与HindIII G的四个BamHI片段（约5.7 Mdal）杂交。用BamHI消化dDNA获得的2.6至3.2-Mdal片段簇似乎代表了与HindIII G中3.0-Mdal BamHI片段相邻的dDNA末端与HSV-1 DNA末端的2.0至2.3-Mdal BamHI片段之间的一个新连接点。
