碘化有机化合物在病毒密度梯度离心法中的应用。

Use of iodinated organic compounds for the density gradient centrifugation of viruses.

作者信息

Gschwender H H, Rutter G, Popescu M

出版信息

Arch Virol. 1975;49(4):359-64. doi: 10.1007/BF01318245.

Abstract

Use of Urografin and Conray for the equilibrium centrifugation of viruses is described. These pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. Poliovirus, Newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to equilibrium in gradients made of these substances. Viral infectivities were not measurably affected, which is especially noteworthy in the case of the very labile lymphocytic choriomeningitis virus. Buoyant densities were found to be significantly lower than densities obtained with gradients made of CsCl and sucrose.

摘要

本文描述了使用泛影葡胺和碘酞葡胺进行病毒平衡离心的方法。这些由碘化芳族化合物组成的药物，密度可达1.6 g/cm³，特性粘度低。脊髓灰质炎病毒、新城疫病毒和淋巴细胞性脉络丛脑膜炎病毒在由这些物质制成的梯度中离心至平衡。病毒感染性未受到可测量的影响，这在非常不稳定的淋巴细胞性脉络丛脑膜炎病毒的情况下尤其值得注意。发现浮力密度显著低于用氯化铯和蔗糖制成的梯度所获得的密度。