一种改进的唾液酸酶纯化方法。
An improved method for purifying sialidase.
作者信息
Geisow M J
出版信息
Biochem J. 1975 Oct;151(1):181-3. doi: 10.1042/bj1510181.
Abstract
An adsorbent specific for sialidase (EC 3.2.1.18) was prepared by coupling a glycoprotein containing glycosidically linked sialic acid to Sepharose. This adsorbent does not display the non-specific adsorption that occurs in previously reported methods.
摘要
通过将含有糖苷键连接的唾液酸的糖蛋白偶联到琼脂糖上，制备了一种对唾液酸酶（EC 3.2.1.18）具有特异性的吸附剂。这种吸附剂不会出现先前报道方法中存在的非特异性吸附。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.N-乙酰神经氨酸的定量测定方法及其在唾液酸粘蛋白水解产物中的应用。
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The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
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Glycoproteins.糖蛋白
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