[高效液相色谱法同时测定不同产地金银花和山银花中6种有机酸]
[Simultaneous determination of six organic acids in Lonicerae japonicae flos and Lonicerae flos in different habitats by HPLC].
作者信息
Liu Min-Yan, Gao Shu-Li, Liu Li-Hua, Zhang Yang, Nian Li-Jun, Liu Xiao-Yan
机构信息
Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang 050035, China.
出版信息
Zhong Yao Cai. 2013 Feb;36(2):196-8.
OBJECTIVE
To establish an HPLC method for simultaneous determination of six organic acids in Lonicerae japonicae Flos and Lonicerae Flos in different habitats.
METHODS
The chromatographic separation was carried out on a Waters C18 column (4. 6 mm x 250 mm, 5 microm) with gradient elution of acetonitrile and 0.1% phosphoric acid at a flow rate of 1 mL/min. The detection wave-length was 326 nm.
RESULTS
The contents of neochlorogenic acid and cryptochlorogenic acid in Lonicerae japonicae Flos in different habitats were very low. The contents of chlorogenic acid in Lonicerae japonicae Flos in Pingyi, Fei County, Fengqiu and Jvlu were significantly higher than that in Shangluo, but the contents of other organic acids were similar. The contents of six kinds of organic acids in Lonicerae Flos in different origins were obviously higher than those in Lonicerae japonicae Flos.
CONCLUSION
A simple, accurate and credible method is developed and validated for quality control of six organic acids from Lonicerae japonicae Flos and Lonicerae Flos.
目的
建立同时测定不同产地金银花和山银花中6种有机酸的高效液相色谱法。
方法
采用Waters C18柱（4.6 mm×250 mm，5μm），以乙腈和0.1%磷酸为流动相进行梯度洗脱，流速为1 mL/min，检测波长为326 nm。
结果
不同产地金银花中新绿原酸和隐绿原酸含量极低。平邑、费县、封丘和巨鹿金银花中绿原酸含量显著高于商洛金银花，但其他有机酸含量相近。不同产地山银花中6种有机酸含量明显高于金银花。
结论
建立了一种简便、准确、可靠的方法，可用于金银花和山银花中6种有机酸的质量控制。
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