乙二胺四乙酸钾对全血中甲状旁腺激素稳定性的影响。

The effect of potassium EDTA on the stability of parathyroid hormone in whole blood.

作者信息

English Emma, McFarlane Ian, Taylor Kevin P, Halsall David J

机构信息

Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge CB2 2QQ, UK.

出版信息

Ann Clin Biochem. 2007 May;44(Pt 3):297-9. doi: 10.1258/000456307780480927.

DOI:10.1258/000456307780480927

PMID:17456300

Abstract

BACKGROUND

The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples.

METHODS

Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium EDTA tubes (EDTA-Ts) and incubated at room temperature before separation and storage for PTH analysis using the Bayer Advia Centaur.

RESULTS

Initial PTH concentrations were 7% higher (95% confidence interval [CI] 2-16%, P=0.016) in EDTA-Ts compared to the SSTs in the renal cohort. PTH concentrations in SSTs were significantly lower after 8 (10%, 95% CI 3-17%) and 20 h (22%, 95% CI 11-31%) but did not change significantly in EDTA-Ts (P=0.1543). PTH concentration in EDTA-Ts from healthy volunteers was also independent of incubation time (P=0.1165).

CONCLUSION

The greater stability of PTH in whole blood anticoagulated with potassium EDTA allows PTH analysis to be offered to sites such as satellite clinics and primary care sites which do not have centrifugation and refrigeration facilities.

摘要

背景

甲状旁腺激素（PTH）在全血中的稳定性尚未得到充分表征。先前的研究表明，乙二胺四乙酸（EDTA）血浆样本比血清样本更稳定。

方法

采集17名血液透析患者和5名健康志愿者的血液，分别置于血清分离管（SST）和乙二胺四乙酸钾管（EDTA-T）中，在室温下孵育，然后分离并储存，使用拜耳Advia Centaur分析仪进行PTH分析。

结果

在肾脏疾病队列中，与SST相比，EDTA-T中的初始PTH浓度高7%（95%置信区间[CI]2-16%，P=0.016）。8小时（10%，95%CI 3-17%）和20小时（22%，95%CI 11-31%）后，SST中的PTH浓度显著降低，但EDTA-T中的PTH浓度无显著变化（P=0.1543）。健康志愿者EDTA-T中的PTH浓度也与孵育时间无关（P=0.1165）。