Kasacka I, Szarmach I J, Buczko P, Tankiewicz A, Pawlak D
Department of Histology and Embryology, Medical University of Białystok, Poland.
Adv Med Sci. 2006;51 Suppl 1:52-4.
In recent years, many reports have focused on clinical changes in the oral cavity of orthodontic patients, manifested in general inflammation of the mucosa. In order to better understand histopathological alterations in the mouth and the use of easily available diagnostic material, we decided to assess the morphology of salivary cells at different time points of treatment with orthodontic appliances.
The study material included non-stimulated saliva obtained from 21 orthodontic patients and 11 healthy secondary school students (controls). After fixation in 96% ethanol the smears were stained with PAS + hematoxylin or H+E, and using the methods of May-Grünwald-Giemsa and Feulgen.
As revealed by the histopathological examinations of saliva smears, patients treated with intra-oral fixed orthodontic appliances showed morphological changes in oral epithelial cells and in the number of leukocytes as compared to the control group. The changes were most pronounced in the first months of treatment.
The preliminary data indicate that orthodontic patients develop changes in the composition and morphology of salivary cells, the intensity of which depends on the time of exposure to the appliance.
近年来，许多报告聚焦于正畸患者口腔的临床变化，表现为黏膜的一般性炎症。为了更好地了解口腔内的组织病理学改变以及易于获取的诊断材料的使用情况，我们决定评估正畸矫治器治疗不同时间点唾液细胞的形态。
研究材料包括从21名正畸患者和11名健康中学生（对照组）获取的非刺激性唾液。在96%乙醇中固定后，涂片用PAS +苏木精或苏木精-伊红染色，并采用美-格-姬氏染色法和福尔根染色法。
唾液涂片的组织病理学检查显示，与对照组相比，接受口腔内固定正畸矫治器治疗的患者口腔上皮细胞和白细胞数量出现形态学变化。这些变化在治疗的最初几个月最为明显。
初步数据表明，正畸患者唾液细胞的组成和形态会发生变化，其变化强度取决于接触矫治器的时间。
