Rizvi Syed Ibrahim, Maurya Pawan Kumar
Department of Biochemistry, University of Allahabad, Allahabad-211002, India.
Ann N Y Acad Sci. 2007 Apr;1100:373-82. doi: 10.1196/annals.1395.041.
The correlation between antioxidant capacity and oxidative damage during aging has been reported in several tissues in different species; however, data on changes of oxidative stress markers in plasma and erythrocytes of healthy populations during aging are few and sometimes contradictory. Since antioxidant capacity of the plasma is related to dietary intake of antioxidants, it is important to study the correlation between antioxidant capacity of the plasma and markers of oxidative stress in different populations. In the present study we report the age-dependent alteration in erythrocyte malondialdehyde (MDA), intracellular-reduced glutathione (GSH), and membrane sulphydryl (SH) groups in the Indian population and also correlate these parameters with total plasma antioxidant potential. We show an age-dependent increase in erythrocyte MDA level and a decrease in GSH and membrane -SH group concentration. The alterations in these parameters correlated significantly with total antioxidant capacity of the plasma. We observe a higher oxidative stress in the Indian population compared to values reported for European subjects emphasizing the need to establish age-dependent reference values for oxidative stress markers in different populations and in studies involving their role in different disease conditions.
衰老过程中抗氧化能力与氧化损伤之间的相关性已在不同物种的多个组织中得到报道；然而，关于健康人群衰老过程中血浆和红细胞氧化应激标志物变化的数据很少，且有时相互矛盾。由于血浆的抗氧化能力与抗氧化剂的饮食摄入量有关，因此研究不同人群血浆抗氧化能力与氧化应激标志物之间的相关性很重要。在本研究中，我们报告了印度人群红细胞丙二醛（MDA）、细胞内还原型谷胱甘肽（GSH）和膜巯基（SH）基团随年龄的变化，并将这些参数与血浆总抗氧化能力相关联。我们发现红细胞MDA水平随年龄增加，而GSH和膜-SH基团浓度降低。这些参数的变化与血浆总抗氧化能力显著相关。与欧洲受试者报告的值相比，我们观察到印度人群的氧化应激更高，这强调了在不同人群以及涉及它们在不同疾病状况中作用的研究中，需要建立氧化应激标志物的年龄依赖性参考值。
