Lopez Ricardo, Dugo Paola, Mondello Luigi
Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, Zaragoza, Spain.
J Sep Sci. 2007 Mar;30(5):669-72. doi: 10.1002/jssc.200600300.
A new method for the quantitative determination of trans-resveratrol in wine has been optimized and validated. The method is based on the direct injection of 500 nL of wine in an HPLC system fitted with an RP microcolumn of 10 cm x 0.32 mm and spectrofluorometric detection. The linear dynamic range of the method covers the normal range of occurrence of the analyte in wines and extends for two orders of magnitude with r(2)= 0.9994. Twenty-three wines have been analyzed with the proposed method, finding concentrations in the range reported by other authors.
一种用于定量测定葡萄酒中反式白藜芦醇的新方法已得到优化和验证。该方法基于将500 nL葡萄酒直接注入配备10 cm×0.32 mm反相微柱的高效液相色谱系统，并进行荧光光谱检测。该方法的线性动态范围涵盖了葡萄酒中分析物的正常存在范围，并延伸了两个数量级，r(2)=0.9994。已用所提出的方法分析了23种葡萄酒，发现其浓度在其他作者报告的范围内。
