Arnold E K, Lovell R A, Beasley V R, Parker A J, Stedelin J R
Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Vet Hum Toxicol. 1991 Oct;33(5):457-61.
Eighteen English pointer dogs were randomly assigned to 3 outdoor grass-plot enclosures (6/enclosure) uniformly sprayed once with either the 2,4-dichlorophenoxyacetic acid (2,4-D) dimethylamine formulation (DMA-4) at the maximum recommended application rate, DMA-4 at 4 times the maximum recommended application rate, or the DMA-4 vehicle alone at 4 times the maximum recommended application rate. A heavy rain shower occurred 24 h after application. The dogs were observed for clinical signs and evaluated using an electroencephalograph, electrocardiograph (lead I), and electromyograph prior to exposure, and either 1 or 7 d after continuous exposure. Clinical examination, hematologic and serum biochemical data were obtained, and serum, urine and kidney 2,4-D were quantified. Half of the dogs from each group were killed after 1 d of continuous exposure, while the other half were killed after 7 d. Gross postmortem and histologic examinations were conducted on each dog. No obvious 2,4-D effects were detected in any of the dogs.
18只英国指示犬被随机分配到3个户外草地围栏中（每个围栏6只），每个围栏均以最大推荐施用量、4倍最大推荐施用量的2,4-二氯苯氧基乙酸（2,4-D）二甲胺制剂（DMA-4）或仅以4倍最大推荐施用量的DMA-4载体进行一次均匀喷洒。喷洒后24小时出现了一场大雨。在暴露前以及连续暴露1天或7天后，对犬进行临床症状观察，并使用脑电图仪、心电图仪（导联I）和肌电图仪进行评估。获取临床检查、血液学和血清生化数据，并对血清、尿液和肾脏中的2,4-D进行定量分析。每组一半的犬在连续暴露1天后处死，另一半在7天后处死。对每只犬进行大体尸检和组织学检查。在任何一只犬中均未检测到明显的2,4-D效应。
