接受全景X线摄影患者颊上皮细胞的微核试验
Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography.
作者信息
Popova L, Kishkilova D, Hadjidekova V B, Hristova R P, Atanasova P, Hadjidekova V V, Ziya D, Hadjidekov V G
机构信息
Laboratory Radiation Genetics, National Centre of Radiobiology and Radiation Protection, Sofia 1756, Bulgaria.
出版信息
Dentomaxillofac Radiol. 2007 Mar;36(3):168-71. doi: 10.1259/dmfr/29193561.
OBJECTIVES
To evaluate the possible genotoxic effect of radiation exposure for dental diagnostic purposes as measured by the formation of micronuclei.
METHODS
The micronucleus test was applied to buccal epithelium cells, which are target cells for dental radiography. Specimens of exfoliated buccal cells were collected from patients subjected to panoramic radiography. Samples were obtained from 32 patients, 12 male and 20 female, aged from 24 years to 73 years, before and 10+/-2 days after panoramic radiation exposure.
RESULTS
No significant increase in the frequency of cells with micronuclei and total number of micronuclei after panoramic tomography was detected. Mean values of buccal cells with micronuclei+/-standard deviation (SD) before and after radiation examination were 2.34+/-1.49% and 2.81+/-1.64%, respectively. A significant correlation between the age of investigated subjects and the initial frequency of micronuclei in buccal cells was observed (r=0.60, P<0.01).
CONCLUSION
Panoramic radiographic examination does not induce micronuclei in target buccal epithelium cells.
目的
通过微核形成情况评估牙科诊断性辐射暴露可能产生的遗传毒性作用。
方法
微核试验应用于口腔颊黏膜上皮细胞，这些细胞是牙科X线摄影的靶细胞。从接受全景X线摄影的患者中收集脱落的颊黏膜细胞标本。样本取自32例患者，年龄24岁至73岁，其中男性12例，女性20例，在全景辐射暴露前及暴露后10±2天采集。
结果
全景断层扫描后，微核细胞频率和微核总数均未显著增加。辐射检查前后颊黏膜微核细胞的平均值±标准差（SD）分别为2.34±1.49%和2.81±1.64%。观察到受调查对象年龄与颊黏膜细胞微核初始频率之间存在显著相关性（r = 0.60，P < 0.01）。
结论
全景X线摄影检查不会在靶颊黏膜上皮细胞中诱导形成微核。
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