Palla Santosh, Rangdhol Vishwanath, Uma A N, Devy Santha A, Shekar Vandana
Department of Oral Medicine and Radiology, KIMS Dental College and Hospital, NH-216, Chaitanya Health City, Amalapuram, Andhra Pradesh, India.
Department of Oral Medicine and Radiology, Vishwanth Dental and oral Medicine Clinic, Bangalore, India.
J Cytol. 2020 Oct-Dec;37(4):189-192. doi: 10.4103/JOC.JOC_120_19. Epub 2020 Sep 16.
Diagnostic radiation is reported to cause significant damage in buccal cells, while the same effects after natural cell turn over cycle were not checked for in previous studies. The buccal cells were studied in patients exposed to computed tomography (CT) scans for evaluating the cells with micronuclei and cytotoxic changes, namely, pyknotic cells, karyorrhectic cells and karyolytic cells. The pre-exposure counts were compared with postexposure counts on 10 and 20 days corresponding to first and second cell turnover cycles.
The aim of this study is to estimate the counts of micronucleus and cytotoxic changes in buccal cells post-exposure to CT scans and report on variance of the same with first and second buccal cell turnover cycles.
This is an observational study, wherein the buccal smears of patients undergoing CT scans were made before and after CT scan exposures as needed. Papanicolaou (PAP) staining and analysis were performed as per standard criteria for micronuclear and cytotoxic changes, respectively. Statistical test used was paired -tests.
The micronuclear counts revealed 0.4% positive cells before exposure and 1.4% positive cells post 10 days and 20 days of exposure were significant ( < 0.005). The cytotoxic changes showed around 2.5% positive cells before and 5.7% positive cells 10 days after CT exposure ( < 0.005). The cytotoxic cell values from baseline to 20 day were not significant ( < 0.25).
CT scans have caused genotoxic effects notable after two cell turnover cycles but the cytotoxic changes have significantly decreased naturally after 2 cell turnover as per our study.
据报道，诊断性辐射会对颊细胞造成显著损伤，而以往研究未检测自然细胞更新周期后的相同效应。对接受计算机断层扫描（CT）的患者的颊细胞进行研究，以评估具有微核和细胞毒性变化的细胞，即固缩细胞、核溶解细胞和核碎裂细胞。将暴露前的计数与暴露后第10天和第20天（对应第一个和第二个细胞更新周期）的计数进行比较。
本研究的目的是估计CT扫描后颊细胞中微核和细胞毒性变化的计数，并报告其在第一个和第二个颊细胞更新周期中的差异。
这是一项观察性研究，根据需要在CT扫描前后对接受CT扫描的患者进行颊涂片。分别按照微核和细胞毒性变化的标准标准进行巴氏（PAP）染色和分析。使用的统计检验是配对检验。
微核计数显示，暴露前阳性细胞为0.4%，暴露后10天和20天阳性细胞为1.4%，差异有统计学意义（P<0.005）。细胞毒性变化显示，CT暴露前阳性细胞约为2.5%，暴露后10天阳性细胞为5.7%（P<0.005）。从基线到20天的细胞毒性细胞值差异无统计学意义（P<0.25）。
根据我们的研究，CT扫描在两个细胞更新周期后会引起明显的遗传毒性效应，但细胞毒性变化在2个细胞更新后自然显著降低。
