Health Services Vocational College, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey.
Faculty of Medicine, Department of Medical Biology, Çanakkale Onsekiz Mart University, Terzioglu Campus, 17100, Çanakkale, Turkey.
Environ Sci Pollut Res Int. 2019 Jul;26(19):19676-19683. doi: 10.1007/s11356-019-05249-0. Epub 2019 May 11.
Since many different pesticides have been used occupationally, there have been inconsistent results regarding DNA damages among greenhouse workers. Thus, the aim of the study is to evaluate DNA damages, cell death, and chromosomal instability by using the buccal micronucleus cytome (BMcyt) assay in greenhouse workers and to compare those with a non-exposed group. The BMcyt assay was applied to the exfoliated buccal cell samples collected from 66 pesticide-exposed and 50 non-exposed individuals. We evaluated the frequency of micronucleus (MN), nuclear bud (NBUD), binucleated (BN) cells, and karyolitic (KL), pyknotic (PY), and karyorrhectic (KH) cells. The results showed that the MN, BN, PY, and KH frequencies of the pesticide-exposed group were significantly higher than those of the controls (P ˂ 0.05, P ˂ 0.05, P ˂ 0.01, and P ˂ 0.05, respectively). We observed that the MN, BN, PY, and KH frequencies in the autumn were statistically different compared with those in the control group (P = 0.037 for MN, P = 0.001 for BN, P = 0.016 for PY, and P = 0.033 for KH). The same comparison was done in the spring for the control, and there was a statistically significant difference for MN (P = 0.046) and PY (P = 0.014). We can conclude that pesticide exposure in greenhouse workers was one of the factors that altered DNA damages, cell death, and chromosomal instability in oral mucosa cells.
由于职业上使用了许多不同的农药，因此温室工作者的 DNA 损伤结果并不一致。因此，本研究的目的是通过颊黏膜微核细胞（BMcyt）试验评估温室工作者的 DNA 损伤、细胞死亡和染色体不稳定性，并将其与非暴露组进行比较。BMcyt 试验应用于从 66 名接触农药和 50 名非接触个体采集的脱落颊细胞样本。我们评估了微核（MN）、核芽（NBUD）、双核（BN）细胞以及核溶解（KL）、固缩（PY）和核碎裂（KH）细胞的频率。结果表明，暴露组的 MN、BN、PY 和 KH 频率明显高于对照组（P<0.05、P<0.05、P<0.01 和 P<0.05）。我们观察到秋季 MN、BN、PY 和 KH 频率与对照组相比存在统计学差异（MN 为 P=0.037，BN 为 P=0.001，PY 为 P=0.016，KH 为 P=0.033）。在春季，我们对对照组进行了同样的比较，MN（P=0.046）和 PY（P=0.014）存在统计学差异。我们可以得出结论，温室工作者接触农药是改变口腔黏膜细胞 DNA 损伤、细胞死亡和染色体不稳定性的因素之一。
