Gercek Arzu, Yildirim Ozlem, Konya Deniz, Bozkurt Suheyla, Ozgen Serdar, Kilic Turker, Sav Aydin, Pamir Necmettin
Department of Anesthesiology and Reanimation, Institute of Neurological Sciences, Marmara University, Maltepe, Istanbul, Turkey.
JPEN J Parenter Enteral Nutr. 2007 May-Jun;31(3):161-6. doi: 10.1177/0148607107031003161.
The aim was to assess wound healing when parenteral fish-oil emulsion is given to rats receiving dexamethasone.
For 5 days after skin wounding, group S (control; n = 7) received saline 1 mL/kg intraperitoneal (IP); group D (n = 7), dexamethasone 0.2 mg/kg IP; and group DO (n = 9), dexamethasone 0.2 mg/kg IP plus 1 mL/kg Omegaven (Fresenius Kabi, Austria). Wound specimens were assessed for hydroxyproline level, wound depth, histology (epidermal/dermal regeneration, granulation tissue thickness, and angiogenesis), and expression of transforming growth factor-beta (TGF-beta) and platelet-derived growth factor-AA (PDGF-AA).
Compared with D and DO specimens, controls had higher hydroxyproline (p < .01), deeper wounds (p < .05), and better histologic scores (p < .01 angiogenesis; others p < .05). There were no significant differences between the group D and DO means for hydroxyproline level, wound depth, or histologic scores (p > .05 for all). Controls had higher TGF-beta expression scores than the other groups (p < .01 for both) and a higher PDGF-AA expression score than group DO (p < .01). Groups D and DO had statistically similar TGF-beta scores, but group D had a higher PDGF-AA score (2.71 +/- 0.75 vs 1.55 +/- 0.72, respectively; p < .05).
According to the parameters we studied, adding parenteral omega-3 and omega-6 fatty acids to the nutrition regimen of rats treated with dexamethasone does not seem to have adverse effects on wound healing, and effects on wound healing may not need to be considered when determining if these agents should be supplemented in nutrition support regimens.
目的是评估给接受地塞米松治疗的大鼠注射肠外鱼油乳剂时的伤口愈合情况。
皮肤损伤后5天，S组（对照组；n = 7）腹腔注射（IP）1 mL/kg生理盐水；D组（n = 7），腹腔注射0.2 mg/kg地塞米松；DO组（n = 9），腹腔注射0.2 mg/kg地塞米松加1 mL/kg Omegaven（奥地利费森尤斯卡比公司）。评估伤口标本的羟脯氨酸水平、伤口深度、组织学（表皮/真皮再生、肉芽组织厚度和血管生成）以及转化生长因子-β（TGF-β）和血小板衍生生长因子-AA（PDGF-AA）的表达。
与D组和DO组标本相比，对照组的羟脯氨酸水平更高（p < 0.01），伤口更深（p < 0.05），组织学评分更好（血管生成p < 0.01；其他p < 0.05）。D组和DO组在羟脯氨酸水平、伤口深度或组织学评分方面的均值无显著差异（所有p > 0.05）。对照组的TGF-β表达评分高于其他组（两者均p < 0.01），PDGF-AA表达评分高于DO组（p < 0.01）。D组和DO组的TGF-β评分在统计学上相似，但D组的PDGF-AA评分更高（分别为2.71±0.75和1.55±0.72；p < 0.05）。
根据我们研究的参数，在接受地塞米松治疗的大鼠营养方案中添加肠外ω-3和ω-6脂肪酸似乎对伤口愈合没有不利影响，在确定营养支持方案中是否应补充这些药物时，可能无需考虑对伤口愈合的影响。
