Koiram Prabhakar R, Veerapur Veeresh P, Kunwar Amit, Mishra Beena, Barik Atanu, Priyadarsini Indira K, Mazhuvancherry Unnikrishnan K
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal- 576 104.
J Radiat Res. 2007 May;48(3):241-5. doi: 10.1269/jrr.06103. Epub 2007 Apr 27.
The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before gamma-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.
研究了姜黄素与单核铜（II）以1:1化学计量比形成的配合物（以下简称配合物）在γ射线照射（4.5 Gy）前30分钟给药，与50 mg/kg剂量的姜黄素相比，对肝脏中抗氧化剂和硫代巴比妥酸反应性物质（TBARS）水平变化的影响。使用比色法在照射后不同时间间隔（1、2和4小时）切除的肝脏匀浆中估计不同的抗氧化剂，如谷胱甘肽（GSH）、谷胱甘肽S-转移酶（GST）、过氧化氢酶、超氧化物歧化酶（SOD）、TBARS和总硫醇。照射后1小时，所有研究的酶水平均出现辐射诱导的下降，而在随后的时间点观察到升高。在假照射小鼠中，姜黄素和配合物处理均降低了GSH和总硫醇水平，而与正常对照相比，过氧化氢酶、GST和SOD水平升高。在辐射影响下，姜黄素和配合物处理均保护了GSH、GST、SOD、过氧化氢酶和总硫醇水平的下降，并抑制了辐射诱导的脂质过氧化。此外，与姜黄素处理组相比，发现配合物在照射后1小时对酶的保护作用更有效。这可能是由于配合物与姜黄素相比，与各种自由基反应的速率常数更高。
