Kaul Zeenia, Yaguchi Tomoko, Harada Jun I, Ikeda Yutaka, Hirano Takashi, Chiura Hiroshi X, Kaul Sunil C, Wadhwa Renu
International Christian University, Division of Natural Sciences, 3-10-2 Osawa, Mitaka, Tokyo 181-8585, Japan.
Biochem Cell Biol. 2007 Feb;85(1):133-40. doi: 10.1139/o06-205.
Quantum dots (QD) are fluorescent semiconductor nanocrystals that are emerging as superior alternatives to the conventional organic dyes used in biological applications. Although QDs offer several advantages over conventional fluorescent dyes, including greater photostability and a wider range of excitation and (or) emission wavelengths, their toxicity has been an issue in its wider use as an analytic, diagnostic and therapeutic tool. We prepared a conjugate QD with an internalizing antibody and demonstrated that the QD-antibody conjugate is efficiently internalized into cells and is visible even after multiple divisions. We demonstrate that the internalized QD is nontoxic to cells and provides a sensitive tool for long-term molecular imaging.
量子点（QD）是荧光半导体纳米晶体，正逐渐成为生物应用中传统有机染料的优质替代品。尽管量子点相较于传统荧光染料具有若干优势，包括更高的光稳定性以及更宽的激发和（或）发射波长范围，但其毒性一直是限制其作为分析、诊断和治疗工具广泛应用的一个问题。我们制备了一种与内化抗体结合的量子点，并证明该量子点 - 抗体共轭物能有效地内化进入细胞，甚至在多次细胞分裂后仍可见。我们证明内化的量子点对细胞无毒，并为长期分子成像提供了一种灵敏的工具。
