Nakade Yukiomi, Mantyh Christopher, Pappas Theodore N, Takahashi Toku
Department of Surgery, Duke University Medical Center and Durham Veterans Affairs Medical Center, Durham, NC, USA.
J Gastroenterol. 2007 Apr;42(4):279-82. doi: 10.1007/s00535-006-1947-2. Epub 2007 Apr 26.
Fecal pellet output has been assessed as a colonic motor activity because of its simplicity. However, it remains unclear whether an acceleration of colonic transit correlates well with an increase in fecal pellet output. We examined the causal relationship between colonic transit and fecal pellet output stimulated by the central application of corticotropin-releasing factor (CRF) and restraint stress.
Immediately after intracisternal injection of CRF, (51)Cr was injected via a catheter positioned in the proximal colon. Ninety minutes after (51)Cr injection, the total number of excreted feces was counted, and then the rats were killed. The radioactivity of each colonic segment was evaluated, and the geometric center (GC) of the distribution of (51)Cr was calculated. For the restraint stress study, after administration of (51)Cr into the proximal colon, rats were submitted to wrapping restraint stress for 90 min. Then they were killed, and GC was calculated.
Both restraint stress and CRF significantly accelerated colonic transit. There was a positive correlation observed between fecal pellet output and GC of colonic transit in response to restraint stress, but not CRF, when the number of excreted feces was more than three. In contrast, there was no significant correlation observed between the two in stress and CRF when the number of excreted feces was less than two.
The acceleration of colonic transit in response to restraint stress and central administration of CRF does not always correlate with an increase in fecal pellet output.
由于粪便颗粒排出量评估方法简单，一直被用作结肠运动活性的评估指标。然而，结肠转运加速是否与粪便颗粒排出量增加密切相关仍不明确。我们研究了促肾上腺皮质激素释放因子（CRF）中枢给药及束缚应激刺激下结肠转运与粪便颗粒排出量之间的因果关系。
脑池内注射CRF后，立即经置于近端结肠的导管注射（51）Cr。注射（51）Cr 90分钟后，计数排出粪便的总数，然后处死大鼠。评估各结肠段的放射性，并计算（51）Cr分布的几何中心（GC）。对于束缚应激研究，在近端结肠注入（51）Cr后，将大鼠进行包裹束缚应激90分钟。然后处死大鼠并计算GC。
束缚应激和CRF均显著加速结肠转运。当排出粪便数量超过3时，束缚应激而非CRF刺激下，粪便颗粒排出量与结肠转运GC呈正相关。相反，当排出粪便数量少于2时，应激和CRF刺激下二者无显著相关性。
束缚应激和CRF中枢给药引起的结肠转运加速并不总是与粪便颗粒排出量增加相关。
