Bosari S, Wiley B D, Hamilton W M, Dugan J M, Cook L B, Lee A K
Department of Anatomic Pathology, Lahey Clinic Medical Center, Burlington, Massachusetts 01805.
Am J Clin Pathol. 1991 Dec;96(6):698-703. doi: 10.1093/ajcp/96.6.698.
Measurement of cellular DNA content may provide useful prognostic information in several human neoplasms. The DNA content by image analysis of fresh tissue has been compared with flow cytometry with good correlation, but the use of paraffin-embedded tissue for image analysis has not been studied widely. This study reports the DNA content of 54 breast carcinomas and compares the results of image analysis of paraffin-embedded tissue and results of image analysis and flow cytometry of corresponding fresh tumors. Image analysis of paraffin blocks and fresh tumors showed comparable results in 51 tumors (94%), whereas 47 tumors (87%) were similar by all three methods. The DNA indices from image analysis of fresh and paraffin-embedded tumors showed significant correlation by linear regression analysis (r = 0.96; P less than 0.001). Discordances between image analysis of fresh and paraffin-embedded tumors are the result of technical problems, such as staining and tissue preservation. Discordances between image analysis and flow cytometry reflect the advantages and pitfalls of the two techniques. These data suggest that image analysis of paraffin-embedded tissue is a viable technique that will permit the performance of retrospective studies with long-term follow-up data for the evaluation of the prognostic significance of DNA content.
细胞DNA含量的测定可为多种人类肿瘤提供有用的预后信息。通过图像分析新鲜组织的DNA含量已与流式细胞术进行比较，二者具有良好的相关性，但用于图像分析的石蜡包埋组织尚未得到广泛研究。本研究报告了54例乳腺癌的DNA含量，并比较了石蜡包埋组织的图像分析结果与相应新鲜肿瘤的图像分析及流式细胞术结果。石蜡块和新鲜肿瘤的图像分析在51例肿瘤（94%）中显示出可比的结果，而通过所有三种方法分析的47例肿瘤（87%）结果相似。新鲜肿瘤和石蜡包埋肿瘤图像分析得出的DNA指数经线性回归分析显示出显著相关性（r = 0.96；P小于0.001）。新鲜肿瘤与石蜡包埋肿瘤图像分析结果不一致是技术问题所致，如染色和组织保存问题。图像分析与流式细胞术结果不一致反映了这两种技术的优缺点。这些数据表明，石蜡包埋组织的图像分析是一种可行的技术，它将允许利用长期随访数据进行回顾性研究，以评估DNA含量的预后意义。
