Saxena R, Saraya A K, Kotte V K, Singh Y N, Prasad L, Malviya A N
Department of Hematology, All India Institute of Medical Sciences, New Delhi.
Am J Clin Pathol. 1991 Dec;96(6):755-8. doi: 10.1093/ajcp/96.6.755.
Lupus anticoagulant was detected in 205 newly diagnosed, untreated patients with systemic lupus erythematosus by the following tests: kaolin clotting time, activated partial thromboplastin time, plasma prothrombin time, and, in the last 99 patients, by dilute Russell's viper venom time. In 10 patients, lupus anticoagulant was detected by kaolin clotting time prolongation, corrected by inosithin but not by normal plasma; 12 and 6 of them had prolonged activated partial thromboplastin time and partial plasma prothrombin time, respectively. Only 10 patients had a history of recurrent abortions and/or thrombosis, nine of whom had lupus anticoagulant as shown by the kaolin clotting time test. Of the 99 patients studied by all four tests, 9 showed lupus anticoagulant by both kaolin clotting time and dilute Russell's viper venom time; 7 had a history of abortion and/or thrombosis. The dilute Russell's viper venom time test is easy to perform and not affected by inhibitors to factor VIII or IX. It is recommended as a primary screening test for lupus anticoagulant detection in a hospital clinical laboratory.
通过以下检测方法在205例新诊断的未经治疗的系统性红斑狼疮患者中检测狼疮抗凝物：高岭土凝血时间、活化部分凝血活酶时间、血浆凝血酶原时间，对于最后99例患者还检测了稀释蝰蛇毒时间。10例患者通过高岭土凝血时间延长检测到狼疮抗凝物，可被肌醇磷脂纠正但不能被正常血浆纠正；其中12例和6例分别有活化部分凝血活酶时间和部分血浆凝血酶原时间延长。只有10例患者有反复流产和/或血栓形成病史，其中9例通过高岭土凝血时间检测显示有狼疮抗凝物。在接受全部四项检测的99例患者中，9例通过高岭土凝血时间和稀释蝰蛇毒时间均显示有狼疮抗凝物；7例有流产和/或血栓形成病史。稀释蝰蛇毒时间检测操作简便，不受因子VIII或IX抑制剂的影响。建议将其作为医院临床实验室检测狼疮抗凝物的初步筛查试验。
