黑升麻抑制人乳腺癌细胞生长机制的基因表达分析
Gene expression analysis of the mechanisms whereby black cohosh inhibits human breast cancer cell growth.
作者信息
Einbond Linda Saxe, Su Tao, Wu Hsan-Au, Friedman Richard, Wang Xiaomei, Jiang Bei, Hagan Timothy, Kennelly Edward J, Kronenberg Fredi, Weinstein I Bernard
机构信息
Department of Rehabilitation Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
出版信息
Anticancer Res. 2007 Mar-Apr;27(2):697-712.
BACKGROUND
Previous studies indicate that specific extracts and the pure triterpene glycoside actein obtained from black cohosh inhibit growth of human breast cancer cells. Our aim is to identify alterations in gene expression induced by treatment with a methanolic extract (MeOH) of black cohosh.
MATERIALS AND METHODS
We treated MDA-MB-453 human breast cancer cells with the MeOH extract at 40 microg/ml and collected RNA at 6 and 24 h; we confirmed the microarray results with real-time RT-PCR for 18 genes.
RESULTS
At 6 h after treatment there was significant increase in expression of ER stress (GRP78), apoptotic (GDF15), lipid biosynthetic (INSIG1 and HSD17B7) and Phase 1 (CYP1A1) genes and, at 24 h, decrease in expression of cell cycle (HELLS and PLK4) genes.
CONCLUSION
Since the MeOH extract activated genes that enhance apoptosis and repressed cell cycle genes, it may be useful in the prevention and therapy of breast cancer.
背景
先前的研究表明，从黑升麻中提取的特定提取物和纯三萜糖苷actein可抑制人乳腺癌细胞的生长。我们的目的是确定用黑升麻甲醇提取物（MeOH）处理后诱导的基因表达变化。
材料与方法
我们用40微克/毫升的MeOH提取物处理MDA-MB-453人乳腺癌细胞，并在6小时和24小时收集RNA；我们用实时RT-PCR对18个基因验证了微阵列结果。
结果
处理后6小时，内质网应激（GRP78）、凋亡（GDF15）、脂质生物合成（INSIG1和HSD17B7）和第一阶段（CYP1A1）基因的表达显著增加，而在24小时时，细胞周期（HELLS和PLK4）基因的表达下降。
结论
由于MeOH提取物激活了增强凋亡的基因并抑制了细胞周期基因，它可能对乳腺癌的预防和治疗有用。
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