活体内内皮细胞中桩蛋白在肌动蛋白丝上的动态运动。
Dynamic motion of paxillin on actin filaments in living endothelial cells.
作者信息
Hu Ying-Li, Chien Shu
机构信息
Department of Bioengineering and the Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093, USA.
出版信息
Biochem Biophys Res Commun. 2007 Jun 15;357(4):871-6. doi: 10.1016/j.bbrc.2007.04.028. Epub 2007 Apr 17.
Abstract
Our three-dimensional (3-D) images showed that paxillin co-localized on actin filaments as fibrous structures, as well as clusters, in endothelial cells (ECs). In living ECs under flow condition, we monitored concurrently the intracellular dynamics of DsRed2-paxillin and GFP-actin by time-lapse video recording and dual-color fluorescence imaging. The results showed that the dynamic motion of paxillin as fibrous structures was associated with actin filaments, but not with microtubules. Our findings suggest that the actin network plays an important role not only in the assembly/disassembly of paxillin at focal adhesions, but also as a track for the intracellular transport of paxillin, which is involved in signaling pathway.
摘要
我们的三维（3-D）图像显示，桩蛋白在内皮细胞（ECs）中作为纤维状结构以及簇状结构与肌动蛋白丝共定位。在流动条件下的活内皮细胞中，我们通过延时视频记录和双色荧光成像同时监测了DsRed2-桩蛋白和GFP-肌动蛋白的细胞内动态。结果表明，作为纤维状结构的桩蛋白的动态运动与肌动蛋白丝相关，而与微管无关。我们的研究结果表明，肌动蛋白网络不仅在粘着斑处桩蛋白的组装/拆卸中起重要作用，而且作为桩蛋白细胞内运输的轨道，参与信号通路。
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