Hines Murray E, Stabel Judith R, Sweeney Raymond W, Griffin Frank, Talaat Adel M, Bakker Douwe, Benedictus Geart, Davis William C, de Lisle Geoffrey W, Gardner Ian A, Juste Ramon A, Kapur Vivek, Koets Ad, McNair Jim, Pruitt Greg, Whitlock Robert H
Tifton Veterinary Diagnostic and Investigational Laboratory, University of Georgia, Tifton, GA 31793, USA.
Vet Microbiol. 2007 Jun 21;122(3-4):197-222. doi: 10.1016/j.vetmic.2007.03.009. Epub 2007 Mar 27.
An international committee of Johne's disease (JD) researchers was convened to develop guidelines for JD challenge studies in multiple animal species. The intent was to develop and propose international standard guidelines for models based on animal species that would gain acceptance worldwide. Parameters essential for the development of long-term and short-term infection models were outlined and harmonized to provide a "best fit" JD challenge model for cattle, goats, sheep, cervids, and mice. These models will be useful to study host-pathogen interactions, host immunity at the local and systemic level, and for evaluating vaccine candidates and therapeutics. The consensus guidelines herein list by animal species strains of Mycobacterium avium subsp. paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters.
一个约翰氏病（JD）研究人员国际委员会召开会议，以制定针对多种动物物种的JD攻毒研究指南。目的是为基于动物物种的模型制定并提出国际标准指南，使其能在全球范围内获得认可。概述并统一了长期和短期感染模型开发所需的参数，以为牛、山羊、绵羊、鹿和小鼠提供“最佳适配”的JD攻毒模型。这些模型将有助于研究宿主-病原体相互作用、局部和全身水平的宿主免疫，以及评估候选疫苗和治疗方法。本文的共识指南按动物物种列出了所用副结核分枝杆菌亚种的菌株、攻毒剂量、剂量频率、攻毒年龄、攻毒途径、接种物制备、实验动物选择、质量控制、最小实验终点及其他参数。
